chemical

OLDA

O-120

O-120_10 mg

OLDA

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

OLDA is an amide of dopamine (DA) and oleic acid. It is a member of the family of N-acyl-dopamines that are recognized as novel biologically active lipid derivatives of dopamine1.The transient receptor potential vanilloid 1 (TRPV1) channel is a nonselective cation channel mainly expressed in primary sensory neurons and sensory C- and A- fibers. TRPV1 can be activated by physical and chemical stimuli, including noxious heat, protons, vanilloid compounds or endogenous arachidonic acid derivatives such as N-oleoyldopamine (OLDA)2.OLDA has been found in striatal, dorsal spinal cord, and dorsal root ganglia3 and identified as the most selective and potent endogenous TRPV1 activator so far with EC values of 36 nM4. OLDA is increasingly recognized as an important class of signaling molecules affecting pain, inflammation, and tissue injury3. Recent studies have shown that OLDA could have a potential role in treating DA-related disorders, such as Parkinson's disease (PD)5.

Alomone Labs

TRPV1 channels

OLDA (#O-120) is a highly pure, synthetic, and biologically active compound.

An Activator of TRPV1 Channel

417.6

N-oleoyl-dopamine

Synthetic

C26H43NO3.

0.5 nM - 50 µM.

OLDA activates TRPV1 channels with an EC50 ~ 0.36 µM1.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.

Ethanol and DMSO up to 50 mM. Centrifuge all product preparations before use (10000 x g 5 min).

Up to four weeks at 4°C or three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>96%

105955-11-1

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

(Z)-N-[2-(3,4-dihydroxyphenyl)ethyl]octadec-9-enamide.