1-Norokadaone | Alomone Labs N-750 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

1-Norokadaone

catalog :

N-750

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs 1-Norokadaone serves as a negative control for Okadaic Acid in modulating the phophatase activity of PP-2A. - Cells were grown to 70% confluence and serum starved for 18 h. Forin vivophosphatase activity the growth medium was replaced with HENK?s and the cells were preincubated in the presence or absence of 100 nMOkadaic Acid (ammonium salt)(#O-800) (orOkadaic Acid (sodium salt)(#O-900)) and 100 nM1-Norokadaone(#N-750) followed by a 20 min incubation with or without 20 M NEM. The cells were then stimulated with 5 ng/ml PDGF for 30 min. At the end of the incubation cell proteins were resolved by SDS-PAGE and probed with anti-phospho-Akt antibodies.

image 2 :

product information

CAT :

N-750

SKU :

N-750_5 mcg

Product Name :

1-Norokadaone

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

1-Norokadaone is structural analogue of Okadaic acid. This compound is a less active homologue than the Okadaic Acid and Okadaol as inhibitors of Protein Phosphatase 1 and Protein Phosphatase 2A, and is thus widely used as negative control for Okadaic Acid1-3.

Supplier :

Alomone Labs

Target :

Protein Phosphatase 1 and Protein Phosphatase 2A

Long Description :

1-Norokadaone (#N-750) is a highly pure, synthetic, and biologically active compound.

Short Description :

An Inactive Homologue of Okadaic Acid and Okadaol, Inhibitors of Protein Phosphatase 1 and Protein Phosphatase 2A

MW :

758.99

Source :

Synthetic

Molecular formula :

C43H66O11.

Activity :

1-Norokadaone is an inactive analogue of okadaic acid (a PP1 and PP2A inhibitor) even at high concentrations1. It may be used as a negative control vis-Ã -vis okadaic acid.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

Solubility :

DMSO, ethanol, acetone or methanol. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to one week at 4°C or three months at -20°C.Protect from light.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

131204-29-0

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

1-[11-Hydroxy-2-[(E)-4-[4-hydroxy-2-[1-hydroxy-3-(3- methyl-1,7-dioxaspiro[5.5]undecan-2-yl)butyl]-3-methylidenespiro [4a,7,8,8a-tetrahydro-4H-pyrano[3,2-b]pyran-6,5'-oxolane]-2'-yl]but-3-en-2-yl]-4-methyl-1,7-dioxaspiro[5.5]undec-4-en-8-yl]propan-2-one.

more info or order :

Alomone Labs product webpage