NNC 55-0396 dihydrochloride | Alomone Labs N-205 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

NNC 55-0396 dihydrochloride

catalog :

N-205

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Drumm B, Rembetski B, Cobine C, Baker S, Sergeant G, Hollywood M, et al. Ca2+ signalling in mouse urethral smooth muscle in situ: role of Ca2+ stores and Ca2+ influx mechanisms. J Physiol. 2018;596:1433-1466 pubmed publisher

image

image 1 :

Alomone Labs NNC 55-0396 dihydrochloride inhibits CaV3.2 channels heterologously expressed inXenopusoocytes. - A. Time course of CaV3.2 peak current amplitude elicited by 100 ms voltage step from holding potential of -100 mV to -20 mV delivered every 10 seconds. Application of 10 MNNC 55-0396 dihydrochloride(#N-205) inhibits the CaV3.2 current in a reversible manner (indicated by the horizontal bar). B. Representative current traces before and during application of 10 M NNC 55-0396 dihydrochloride as indicated.

image 2 :

Peptide (C)RAKRGRSAESLHMTSE corresponding to amino acid residues 1039-1054 of rat RIM1 (AccessionQ9JIR4). Intracellular.

product information

CAT :

N-205

SKU :

N-205_10 mg

Product Name :

NNC 55-0396 dihydrochloride

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

NNC 55-0396 dihydrochloride, is a structural analog of mibefradil (Ro 40-5967) that inhibits both T-type and high-voltage-activated (HVA) Ca2+ channels with a higher selectivity for T-type Ca2+ channels. NNC 55-0396 exerts no effect against high voltage Ca2+ channels at 100 μM, but inhibits T-type channels in HEK293 cells with a potency comparable to that of mibefradil (IC50 values of 6.8 versus 10.1 μM)1. NNC 55- 0396 can be an essential tool in preventing human ovarian cancer cell proliferation as a result of its ability to inhibit the function of T-type Ca2+ channels. It is believed that NNC 55-0396 may function by dissolving in or passing through the plasma membrane of cells2.

Supplier :

Alomone Labs

Target :

T-type CaV channels

Long Description :

NNC 55-0396 dihydrochloride (#N-205) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Blocker of T-type CaV Channels

MW :

564.57

Source :

Synthetic

Molecular formula :

C30H39.8Cl1.8FN3O2.

Effective Concentration :

1-100 µM.

Activity :

NNC 55-0396 displays IC50 values of 6.8 and >100 μM for inhibition of CaV3.1 T-type channels and HVA currents respectively in INS-1 cells1.

Reconstitution and Solubility :

50 mM in water. Centrifuge all product preparations before use (10000 x g for 1 min).

Solubility :

50 mM in water. Centrifuge all product preparations before use (10000 x g for 1 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>95%

CAS No :

357400-13-6

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(1S,2S)-2-[2-[[3-(1H-Benzimidazol-2

more info or order :

Alomone Labs product webpage