NBQX | Alomone Labs N-185 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

NBQX

catalog :

N-185

more info or order :

Alomone Labs product webpage

citations: 14

Reference
Irie T. Essential Role of Somatic Kv2 Channels in High-Frequency Firing in Cartwheel Cells of the Dorsal Cochlear Nucleus. Eneuro. 2021;8: pubmed publisher
Gruszczynska Biegala J, Strucinska K, Maciąg F, Majewski L, Sladowska M, Kuznicki J. STIM Protein-NMDA2 Receptor Interaction Decreases NMDA-Dependent Calcium Levels in Cortical Neurons. Cells. 2020;9: pubmed publisher
Kang Y, Clement E, Sumsky S, Xiang Y, Park I, Santaniello S, et al. The critical role of persistent sodium current in hippocampal gamma oscillations. Neuropharmacology. 2020;162:107787 pubmed publisher
Spratt P, Ben Shalom R, Keeshen C, Burke K, Clarkson R, Sanders S, et al. The Autism-Associated Gene Scn2a Contributes to Dendritic Excitability and Synaptic Function in the Prefrontal Cortex. Neuron. 2019;: pubmed publisher
Getachew B, Tizabi Y. Both Ketamine and NBQX Attenuate Alcohol-Withdrawal Induced Depression in Male Rats. J Drug Alcohol Res. 2019;8: pubmed publisher
Maciąg F, Majewski Ł, Boguszewski P, Gupta R, Wasilewska I, Wojtas B, et al. Behavioral and electrophysiological changes in female mice overexpressing ORAI1 in neurons. Biochim Biophys Acta Mol Cell Res. 2019;1866:1137-1150 pubmed publisher
Ruda Kucerova J, Babinska Z, Luptak M, Getachew B, Tizabi Y. Both ketamine and NBQX attenuate alcohol drinking in male Wistar rats. Neurosci Lett. 2018;666:175-180 pubmed publisher
Irie T, Trussell L. Double-Nanodomain Coupling of Calcium Channels, Ryanodine Receptors, and BK Channels Controls the Generation of Burst Firing. Neuron. 2017;96:856-870.e4 pubmed publisher
Murphy T, Davila D, Cuvelier N, Young L, Lauderdale K, Binder D, et al. Hippocampal and Cortical Pyramidal Neurons Swell in Parallel with Astrocytes during Acute Hypoosmolar Stress. Front Cell Neurosci. 2017;11:275 pubmed publisher
Gao Y, Budlong C, Durlacher E, Davison I. Neural mechanisms of social learning in the female mouse. elife. 2017;6: pubmed publisher
Zhu J, Lee K, Jewett K, Man H, Chung H, Tsai N. Epilepsy-associated gene Nedd4-2 mediates neuronal activity and seizure susceptibility through AMPA receptors. PLoS Genet. 2017;13:e1006634 pubmed publisher
Libbey J, Hanak T, Doty D, Wilcox K, Fujinami R. NBQX, a highly selective competitive antagonist of AMPA and KA ionotropic glutamate receptors, increases seizures and mortality following picornavirus infection. Exp Neurol. 2016;280:89-96 pubmed publisher
Grimes W, Schwartz G, Rieke F. The synaptic and circuit mechanisms underlying a change in spatial encoding in the retina. Neuron. 2014;82:460-73 pubmed publisher
Quattrocolo G, Maccaferri G. Novel GABAergic circuits mediating excitation/inhibition of Cajal-Retzius cells in the developing hippocampus. J Neurosci. 2013;33:5486-98 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 5) rat testis (lanes 2 and 6) mouse brain (lanes 3 and 7) and mouse testis (lanes 4 and 8): - 1-4.Anti-RXFP2 (extracellular) Antibody(#ARR-012) (1:400).5-8. Anti-RXFP2 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

N-185

SKU :

N-185_10 mg

Product Name :

NBQX

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

The α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPA receptor) is a non-N-methyl-D-aspartate (non-NMDA) type ionotropic transmembrane receptor for glutamate that mediates fast synaptic transmission in the central nervous system (CNS)1. AMPA receptors normally assemble from four genetically distinct subunits: GluR1, GluR2, GluR3, and GluR4, which combine to form receptor complexes with different functional properties2.NBQX (2,3-Dihydroxy-6-nitro-7-sulfamoyl-benzo(F) quinoxaline) is an analogue of the quinoxalinedione antagonists to the non-NMDA glutamate receptor. NBQX is a potent and selective inhibitor of binding to the quisqualate subtype of the glutamate receptor, with no activity at the NMDA and glycine site3. It is a specific and potent AMPA receptor antagonist and has been found to be neuroprotective in various models of ischemia and to have anticonvulsant properties in different models of epilepsy4. NBQX has been reported to block the excitatory actions of AMPA in rat neocortex slices while having no effect on NMDA responses5. It was also reported to selectively block AMPA-induced depolarizations but not spontaneous epileptiform discharges in Mg2+ free medium in the rat cortical wedge6. NBQX blocks AMPA and kainate receptors at micromolar concentrations7,8.

Supplier :

Alomone Labs

Target :

AMPA/Kainate receptors

Long Description :

NBQX (#N-185) is a highly pure, synthetic, and biologically active compound.

Short Description :

An Antagonist of AMPA/Kainate Receptors

MW :

336.3

Source :

Synthetic

Molecular formula :

C12H8N4O6S.

Effective Concentration :

0.1-500 µM.

Activity :

NBQX is a potent inhibitor of non-NMDA glutamate receptor channels exhibiting IC50s of 0.1-0.4 μM for AMPA and 1.7-8 μM for kainate receptors1.

Reconstitution and Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99%

CAS No :

118876-58-7

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide.

more info or order :

Alomone Labs product webpage