NMDA | Alomone Labs N-170 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

NMDA

catalog :

N-170

more info or order :

Alomone Labs product webpage

citations: 16

Reference
Iuliano M, Seeley C, Sapp E, Jones E, Martin C, Li X, et al. Disposition of Proteins and Lipids in Synaptic Membrane Compartments Is Altered in Q175/Q7 Huntington's Disease Mouse Striatum. Front Synaptic Neurosci. 2021;13:618391 pubmed publisher
Fani G, Mannini B, Vecchi G, Cascella R, Cecchi C, Dobson C, et al. Aβ Oligomers Dysregulate Calcium Homeostasis by Mechanosensitive Activation of AMPA and NMDA Receptors. ACS Chem Neurosci. 2021;12:766-781 pubmed publisher
Yeung J, Palpagama T, Tate W, Peppercorn K, Waldvogel H, Faull R, et al. The Acute Effects of Amyloid-Beta1-42 on Glutamatergic Receptor and Transporter Expression in the Mouse Hippocampus. Front Neurosci. 2019;13:1427 pubmed publisher
Avazzadeh S, McDonagh K, Reilly J, Wang Y, Boomkamp S, McInerney V, et al. Increased Ca2+ signaling in NRXN1α +/- neurons derived from ASD induced pluripotent stem cells. Mol Autism. 2019;10:52 pubmed publisher
Shi G, Xing L, Wu D, Bhattacharyya B, Jones C, McMahon T, et al. A Rare Mutation of β1-Adrenergic Receptor Affects Sleep/Wake Behaviors. Neuron. 2019;103:1044-1055.e7 pubmed publisher
Leyrer Jackson J, Thomas M. Layer-specific effects of dopaminergic D1 receptor activation on excitatory synaptic trains in layer V mouse prefrontal cortical pyramidal cells. Physiol Rep. 2018;6:e13806 pubmed publisher
Leyrer Jackson J, Thomas M. Subtype-specific effects of dopaminergic D2 receptor activation on synaptic trains in layer V pyramidal neurons in the mouse prefrontal cortex. Physiol Rep. 2017;5: pubmed publisher
Aloisi E, Le Corf K, Dupuis J, Zhang P, Ginger M, Labrousse V, et al. Altered surface mGluR5 dynamics provoke synaptic NMDAR dysfunction and cognitive defects in Fmr1 knockout mice. Nat Commun. 2017;8:1103 pubmed publisher
Lambert M, Ipsen T, Kohlmeier K. Acute cocaine exposure elicits rises in calcium in arousal-related laterodorsal tegmental neurons. Pharmacol Res Perspect. 2017;5:e00282 pubmed publisher
Roshanravan H, Kim E, Dryer S. NMDA Receptors as Potential Therapeutic Targets in Diabetic Nephropathy: Increased Renal NMDA Receptor Subunit Expression in Akita Mice and Reduced Nephropathy Following Sustained Treatment With Memantine or MK-801. Diabetes. 2016;65:3139-50 pubmed publisher
Leshchyns ka I, Liew H, Shepherd C, Halliday G, Stevens C, Ke Y, et al. AÎ²-dependent reduction of NCAM2-mediated synaptic adhesion contributes to synapse loss in Alzheimer's disease. Nat Commun. 2015;6:8836 pubmed publisher
PaÅŸca A, Sloan S, Clarke L, Tian Y, Makinson C, Huber N, et al. Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture. Nat Methods. 2015;12:671-8 pubmed publisher
Sasmal D, Lu H. Single-molecule patch-clamp FRET microscopy studies of NMDA receptor ion channel dynamics in living cells: revealing the multiple conformational states associated with a channel at its electrical off state. J Am Chem Soc. 2014;136:12998-3005 pubmed publisher
Ari C, Borysov S, Wu J, Padmanabhan J, Potter H. Alzheimer amyloid beta inhibition of Eg5/kinesin 5 reduces neurotrophin and/or transmitter receptor function. Neurobiol Aging. 2014;35:1839-49 pubmed publisher
Chen W, Walwyn W, Ennes H, Kim H, McRoberts J, Marvizon J. BDNF released during neuropathic pain potentiates NMDA receptors in primary afferent terminals. Eur J Neurosci. 2014;39:1439-54 pubmed publisher
Tamura A, Yamada N, Yaguchi Y, Machida Y, Mori I, Osanai M. Both neurons and astrocytes exhibited tetrodotoxin-resistant metabotropic glutamate receptor-dependent spontaneous slow Ca2+ oscillations in striatum. PLoS ONE. 2014;9:e85351 pubmed publisher

image

image 1 :

Alomone Labs NMDA activates NMDA (GluNR1+GluNR2c) receptor channels heterologously expressed inXenopusoocytes. - Continuous current trace of NMDA receptor channel activity at 0 mV holding potential.NMDA(#N-170) was applied at concentrations of 0.1 to 1000 M and the horizontal bars above the trace represent periods of NMDA perfusion.

product information

CAT :

N-170

SKU :

N-170_1 g

Product Name :

NMDA

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

NMDA is the prototypic NMDA receptor agonist. An amino acid that, as a D-isomer, is the defining agonist for the NMDA receptor subtype of glutamate receptors1.NMDA is a water-soluble synthetic substance that is not normally found in biological tissues. It was first synthesized in the 1960s. NMDA is an excitotoxin in high quantities (it kills nerve cells by over-exciting them)2.NMDA activates NMDA receptors by binding to NR2 subunits, leading to the opening of a non-specific cation channel, which can allow the passage of Ca2+ and Na+ ions into the cell and K+ ions out of the cell3.NMDA was evaluated for the inhibition of [3H]MK-801 binding at N-methyl-D-aspartate glutamate receptor and IC50 with an IC50 of 6.86 µM1. Cortical cultures of rat brain neurons have an estimated EC50 value of 6.8 µM4. The EC50 found for NR2 subunit expressed in Xenopus oocytes was 21.7 µM3.

Supplier :

Alomone Labs

Target :

NMDA receptors

Long Description :

NMDA (#N-170) is a highly pure, synthetic, and biologically active compound.

Short Description :

An NMDA Receptor Agonist

MW :

147.1

Source :

Synthetic

Molecular formula :

C5H9NO4.

Effective Concentration :

6.5-500 µM.

Activity :

Prototypic NMDA receptor agonist. An amino acid that, as a D-isomer, is the defining agonist for the NMDA receptor subtype of glutamate receptors1. NMDA is a water-soluble synthetic substance that is not normally found in biological tissues2. EC50 ranges between 6.5-25.0 μM, depending on the tissue applied on1,3,4.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity

Solubility :

Water. Centrifuge all product preparations before use (10000 x g for 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

6384-92-5

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Cited Application :

Electrophysiology

Chemical Name :

N-Methyl-D-aspartic acid.

more info or order :

Alomone Labs product webpage