NS-1619 | Alomone Labs N-105 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

NS-1619

catalog :

N-105

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Echeverry S, Grismaldo A, Sanchez C, Sierra C, Henao J, Granados S, et al. Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration. Front Physiol. 2020;11:210 pubmed publisher
Zhao H, Xue Q, Li C, Wang Q, Han S, Zhou Y, et al. Upregulation of Beta4 subunit of BKCa channels in the anterior cingulate cortex contributes to mechanical allodynia associated anxiety-like behaviors. Mol Brain. 2020;13:22 pubmed publisher
Yang M, James A, Suman R, Kasprowicz R, Nelson M, O Toole P, et al. Voltage-dependent activation of Rac1 by Nav 1.5 channels promotes cell migration. J Cell Physiol. 2020;235:3950-3972 pubmed publisher

image

image 1 :

Alomone Labs NS-1619 enhances KCa1.1 currents inXenopusoocytes. - A. Families of KCa1.1 channel current responses to increasing voltage step stimulation (from -50 mV to + 50 mV) before (left) and during (right) application of 100 ?MNS-1619(#N-105). Currents were elicited by 100 ms voltage step from -100 mV applied every 10 sec. B. Time course of current amplitude at 0 mV before during application of 100 ?M NS-1619 (green) and upon wash demonstrating the current amplitude enhancement. C. Voltage dependence of KCa1.1 channel activity enhancement.

image 2 :

Western blot analysis of human LNCaP prostate carcinoma (lanes 1 and 4) human HeLa cervix carcinoma (lanes 2 and 5) and human HepG2 liver carcinoma (lanes 3 and 6)cell line lysates: - 1-3. Anti-Human ABCB1/MDR1 Antibody(#AMT-021) (1:200).4-6. Anti-Human ABCB1/MDR1 Antibody preincubated with the negative control antigen.

product information

CAT :

N-105

SKU :

N-105_10 mg

Product Name :

NS-1619

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

The large conductance voltage- and Ca2+-activated K+ (BK) channels, also known as BKCa, KCa1.1, Slo, or MaxiK, play an important role in modulating nerve and muscle cell contractility and excitability1-3. BK channels are activated by both depolarizing voltages and by an increase in intracellular free Ca2+, thus coupling the membrane potential to intracellular signaling.NS-1619 is a potent and selective KCa1.1 channel modulator (enhancer)4. It has the potential to modulate cell excitability in neurons and smooth muscle5-7. The effect of relaxation of NS-1619 on the BK channel in smooth muscle cells has an EC50 of 10-30 µM. It is also dose-dependent, resulting in a shift of the activation curve by up to -50 mV towards negative membrane potentials, but fully reversible8.NS-1619 also modulates intracellular Ca2+ by inhibiting SERCA pumps9.

Supplier :

Alomone Labs

Target :

KCa1.1 K+ channels, SERCA pumps

Long Description :

NS-1619 (#N-105) is a highly pure, synthetic, and biologically active compound.

Short Description :

Selective and Potent Enhancer of KCa1.1 Channels and an Inhibitor of SERCA Pumps

MW :

362.2

Source :

Synthetic

Molecular formula :

C15H8F6N2O2.

Effective Concentration :

100 nM - 100 μM.

Activity :

NS-1619 is a potent and selective KCa1.1 (BK, slo, Large conductance Ca2+-activated K+) channel modulator (enhancer)1. NS-1619 was effective in eliciting and enhancing KCa1.1 channels in smooth muscle as well as in neurons with maximal effects at concentrations around 30 μM1,2. NS-1619 was used in vivo by intracarotid infusion and significantly enhanced blood tumor barrier permeability in the brain tumor area but not in normal brain tissue3.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.

Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>95%

CAS No :

153587-01-0

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

1,3-Dihydro-1-[2-hydroxy-5-(trifluoromethyl)phenyl]-5-(trifluoromethyl)-2H-benzimidazol-2-one.

more info or order :

Alomone Labs product webpage