M8-B hydrochloride | Alomone Labs M-220 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

M8-B hydrochloride

catalog :

M-220

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat DRG (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates: - 12.Anti-PeriaxinAntibody (#ANR-091) (1:600).34. Anti-Periaxin Antibody preincubated with the negative control antigen.

product information

CAT :

M-220

SKU :

M-220_10 mg

Product Name :

M8-B hydrochloride

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

M8-B hydrochloride is a potent and selective antagonist of the transient receptor potential melastatin-8 (TRPM8) channel1. It inhibits rat and human TRPM8 channels by cold, by icilin and by menthol and demonstrates IC50 values of 7.8 nM, 26.9 nM, and 64.3 nM, respectively. The compound shows little or no effect on other TRP channels1.TRPM8 is a Ca2+-permeable channel, a member of the 'long' or melastatin (M) group of TRP channels and is activated by temperatures range of 8-28°C or by cooling compounds. The TRPM8 gene is up-regulated in prostate cancer and other malignancies. The channel is specifically expressed in temperature-sensing trigeminal and dorsal root ganglion neurons2,3.

Supplier :

Alomone Labs

Target :

TRPM8 channel

Long Description :

M8-B hydrochloride (#M-220) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Potent and Selective Blocker of TRPM8 Channel

MW :

432.96

Source :

Synthetic

Molecular formula :

C22H25ClN2O3S.

Effective Concentration :

10 nM - 10 µM.

Activity :

M8-B hydrochloride is a potent and selective blocker of TRPM8 channel, inhibiting rat and human TRPM8 activation by cold, by icilin and by menthol with IC50 values of 7.8 nM, 26.9 nM and 64.3 nM, respectively, whilst having little or no effect on other TRP channels (IC50 > 20 μM)1.

Reconstitution and Solubility :

Up to 100 mM in DMSO. Centrifuge all products before use (10000 x g 5 min).

Solubility :

Up to 100 mM in DMSO. Centrifuge all products before use (10000 x g 5 min).

Storage of solutions :

Up to three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99%

CAS No :

883976-12-3

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

N-(2-aminoethyl)-N-[(3-methoxy-4 phenylmethoxyphenyl) methyl]thiophene-2 carboxamide;hydrochloride.

more info or order :

Alomone Labs product webpage