ML 218 | Alomone Labs M-165 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

ML 218

catalog :

M-165

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Grossini E, Gramaglia C, Farruggio S, Camillo L, Mary D, Vacca G, et al. Asenapine modulates nitric oxide release and calcium movements in cardiomyoblasts. J Pharmacol Pharmacother. 2016;7:6-14 pubmed publisher

image

image 1 :

Alomone Labs ML 218 inhibits T-type CaVchannels heterologously expressed inXenopusoocytes. - A. Time course of CaV3.2 peak current amplitude elicited by 100 ms voltage step from holding potential of -100 mV to -30 mV delivered every 10 seconds. Application of 2 MML 218(#M-165) inhibits the CaV3.2 current in a reversible manner (indicated by the horizontal bar). B. Representative current traces before and during application of 2 M ML 218 as indicated.

image 2 :

Expression ofZinc transporter 1 in rat olfactory bulb - Immunohistochemical staining of perfusion-fixed frozen rat brain sectionsusingAnti-ZnT1 (SLC30A1) Antibody(#AZT-011) (1:300) followed by goat anti-rabbit-AlexaFluor-488 antibody. ZnT1 staining (green) appears in glomeruli (G) and is particularly intense in the outer border (arrows). Cell nuclei are labeled with DAPI (blue).

product information

CAT :

M-165

SKU :

M-165_10 mg

Product Name :

ML 218

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

T-Type Ca2+ channel inhibitors hold tremendous therapeutic potential for the treatment of pain, epilepsy, sleep disorders, essential tremor, and other neurological disorders1. ML 218 is a selective, potent inhibitor of T-type Ca2+ channels (IC50 values are 270 and 310 nM for CaV3.3 and CaV3.2 respectively in a patch EP assay). It has no effect on other members of the Ca2+ family of ion channels (L- and N-type Ca2+ channels) as well as multiple Na+ and K+ channels. ML 218 was found to be non-selective against hERG and many other ion channels. ML 218 possess excellent DMPK properties, acceptable in vivo rat PK, excellent brain levels and was efficacious in a haloperidol-induced catalepsy assay, a preclinical Parkinsonian model. ML 218 decreases burst activity in subthalamic nucleus (STN) neurons. Thus, ML 218 is a powerful new probe to study T-Type Ca2+ function both in vitro and in vivo2.

Supplier :

Alomone Labs

Target :

T-type CaV channels

Long Description :

ML 218 (#M-165) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Selective Blocker of T-type Channels

MW :

369.3

Synonyms :

MLS003431854, SMR002112449, VU0413807-1, VU0413807-2, VU0424199-1

Source :

Synthetic

Molecular formula :

C19H26Cl2N2O.

Effective Concentration :

0.1-100 µM.

Activity :

ML 218 is a blocker of T-type Ca2+ channels (IC50 values are 270 and 310 nM for CaV3.3 and CaV3.2 respectively)1.

Reconstitution and Solubility :

25 mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).

Solubility :

25 mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

1346233-68-8

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

3,5-Dichloro-N-[[(1α,5α,6-exo,6α)-3-(3,3-dimethylbutyl)-3-azabicyclo[3.1.0]hex-6-yl]methyl]-benzamide.

more info or order :

Alomone Labs product webpage