chemical

m-Chlorophenylbiguanide hydrochloride

M-160

M-160_1 g

m-Chlorophenylbiguanide hydrochloride

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine neurotransmitter synthesized from the amino acid tryptophan by the enzyme tryptophan-decarboxylase. The 5-HT3 channel is a ligand gated ion channel receptor of the cys-loop channel family. It is a pentamer with five subunits surrounding a central channel1.m-Chlorophenylbiguanide hydrochloride (mCPG) is a prototypical, potent and selective 5-HT3 receptor agonist. In a study conducted in N1E-115 neuroblastoma cells mCPG exhibited inward rectification and reversed currents similar to those induced by 5-HT binding to the 5-HT3 receptor. The rate of receptor desensitization is similar to that of 5-HT binding but the peak current amplitude provoked by the agonist was found to be only 20% at saturating concentration2. Interestingly, mCPG seems to facilitate noradrenaline release in mouse brain cortex cell. When mouse brain slices were incubated with H-noradrenaline, electrical field stimulation was facilitated by mCPG and this effect was blocked by the α2 adrenergic receptor antagonist, Rauwolscine3. mCPG binds and activates recombinant α9/α10 acetylcholine receptors in Xenopus oocytes (although 5-HT does not) thus producing inwards currents that are 3.1±0.7% of the maximum effect obtained by ACh binding4.

Alomone Labs

5-HT3 channels

m-Chlorophenylbiguanide hydrochloride (#M-160) is a highly pure, synthetic, and biologically active compound.

A Potent and Selective Agonist of 5-HT3 Receptors

248.11

mCPG

Synthetic

C8H11Cl2N5.

0.1-20 µM.

m-Chlorophenylbiguanide is an activator/agonist of 5-HT3 channels1.

100 mM in H2O. Centrifuge all product preparations before use (10000 x g 5 min).

100 mM in H2O. Centrifuge all product preparations before use (10000 x g 5 min).

Up to four weeks at 4°C or three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>99%

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Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

2-(3-chlorophenyl)-1-(diaminomethylidene)guanidine;hydrochloride.