chemical

Leptomycin B

L-500

L-500_1 mcg

Leptomycin B

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Leptomycin B is an unsaturated, branched-chain fatty acid antibiotic which produced by Streptomyces sp. ATS1287.1,2 Leptomycin B was found to act as an antifungal antibiotic and reversible antiproliferative agent which arrests the cell cycle in both the G1 and G2 phases into the resting state - GO.3In the late 90's Leptomycin B, was found as inhibitor of the nucleo-cytoplasmic translocation process of the HIV-1 Rev protein and Rev-dependent mRNA at nanomolar concentrations and was the first low molecular weight inhibitor of nuclear export to be identified.4The target molecule of Leptomycin B has been shown by genetic and biochemical analyses to be CRM1, a highly conserved protein in eukaryotes. CRM1 was shown to be a member of the importin beta family and a receptor for the nuclear export signal (NES) of proteins in both yeast and mammalian cells. Leptomycin B binds directly to CRM1, which results in the dissociation of the NES from the nuclear export machinery containing CRM1 and leads to the nuclear accumulation of many proteins in both yeast and mammalian cells.5-7Thus, Leptomycin B serves as a potent tool for understanding the molecular mechanisms of nucleo-cytoplasmic transport of proteins.

Alomone Labs

Leptomycin B (#L-500) is a highly pure, natural, and biologically active compound.

A Protein Synthesis and Transport Inhibitor

540.75

CI 940, LMB, Elactocin, Mantuamycin

Natural

C33H48O6.

1-50 nM.

Leptomycin B, an unsaturated, branched fatty-acid chain, is an antifungal antibiotic1,2 which arrests the cell cycle3 and prevents the nuclear export of proteins4,5.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.

Product is supplied in 100 µl 70% methanol (1 µg supplied at a concentration of 18.5 µM, and 5 µg supplied at a concentration of 92.5 µM). Keep on ice when working to prevent solvent evaporation. Do not dry. Do not use DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Store at -20°C. Protect from light.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>98%

87081-35-4

Contact Alomone Labs for technical support and product customization

41116134

Yes

(2E,5S,6R,7S,9R,10E,12E,15R,16Z,18E)-19-[(2S,3S)-3,6-Di hydro-3-methyl-6-oxo-2H-pyran-2-yl]-17-ethyl-6-hydroxy-3,5,7,9,11,15-hexamethyl-8-oxo-2,10,12,16,18 nonadecapentaenoic acid.