LY 294002 | Alomone Labs L-300 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

LY 294002

catalog :

L-300

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Oh K, Lee O, Park Y, Seo M, Lee D. IL-1? induces IL-6 production and increases invasiveness and estrogen-independent growth in a TG2-dependent manner in human breast cancer cells. BMC Cancer. 2016;16:724 pubmed publisher
Puckett M, Goldman E, Cockrell L, Huang B, Kasinski A, Du Y, et al. Integration of apoptosis signal-regulating kinase 1-mediated stress signaling with the Akt/protein kinase B-I?B kinase cascade. Mol Cell Biol. 2013;33:2252-9 pubmed publisher
Ishima T, Iyo M, Hashimoto K. Neurite outgrowth mediated by the heat shock protein Hsp90?: a novel target for the antipsychotic drug aripiprazole. Transl Psychiatry. 2012;2:e170 pubmed publisher
Graves B, Simerly T, Li C, Williams D, Wondergem R. Phosphoinositide-3-kinase/akt - dependent signaling is required for maintenance of [Ca(2+)](i), I(Ca), and Ca(2+) transients in HL-1 cardiomyocytes. J Biomed Sci. 2012;19:59 pubmed publisher
Egea J, Espinet C, Soler R, Dolcet X, Yuste V, Encinas M, et al. Neuronal survival induced by neurotrophins requires calmodulin. J Cell Biol. 2001;154:585-97 pubmed

image

image 1 :

Alomone Labs Wortmannin inhibits AKT phosphorylation via PI3-kinase in NIH-3T3 cells. - Cells were preincubated for 30 min in different concentrations ofLY 294002(#L-300) orWortmannin(#W-400) and then stimulated with PDGF (3 ng/ml) for 20 min. Cell proteins were resolved by SDS-PAGE and probed with (A) anti-phospho-AKT antibody followed by stripping and reprobed with (B) anti-Akt antibody.

image 2 :

Western blot analysis of human HL-60 promyelocytic leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4): - 12. Anti-Human Adenosine A2AReceptor (extracellular) Antibody (#AAR-007) (1:200).34. Anti-Human Adenosine A2AReceptor (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

L-300

SKU :

L-300_1 mg

Product Name :

LY 294002

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

PI 3-kinase is an enzyme implicated in growth factor signal transduction by associating with receptor and non-receptor tyrosine kinases.1,2 LY-294002 is a potent and specific cell-permeable inhibitor of Pl 3-kinase that acts on the ATP-binding site of the enzyme.3In in vitro assays it displays an IC50 of 1.4 µM on purified PI 3-kinase, but did not inhibit PKC, PKA, MAP kinase and PI 4-kinase.3 In fMet-Leu-Phe stimulated neutrophils, LY-294002 completely abolished PI 3-kinase activity at 50 µM.4 LY-294002 induces apoptosis in many cell types by blocking the PI3-K/Akt anti-apoptotic pathway.5,6 Thus LY-294002 is useful tool for identifying the biochemical events regulated by the PI3-K/Akt pathway.7 This pathway has important relevance to the cellular events involved in cancer.6,8,9

Supplier :

Alomone Labs

Target :

PI3-Kinase

Long Description :

LY 294002 (#L-300) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Specific Inhibitor of PI3-Kinase

MW :

307.35

Source :

Synthetic

Molecular formula :

C19H17NO3.

Effective Concentration :

5-10 µM.

Activity :

LY 294002 is a potent and specific cell-permeable inhibitor of Pl3-kinase1.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.

Solubility :

DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99%

CAS No :

154447-36-6

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

2-(4-Morpholinyl)-8-phenyl-4H-1-benzopyran-4-one hydrochloride.

more info or order :

Alomone Labs product webpage