Lemborexant | Alomone Labs L-255 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Lemborexant

catalog :

L-255

more info or order :

Alomone Labs product webpage

image

image 1 :

ManufacturerBioassayAlomone Labs Lemborexant inhibits human OX1 receptors expressed in CHO-K1 cells. - Dose response curve of the normalized inhibition of hOX1 receptor activity by Lemborexant (#L-255). Cells were loaded with Fluo-8 dye, incubated with increasing concentrations of Lemborexant, and stimulated with 15 nM Orexin-A. Changes in intracellular Ca2+ following agonist application were detected as changes in maximum relative fluorescence (RLU) using FLIPRTETRA™. IC50 was calculated at 58.7 nM.

product information

CAT :

L-255

SKU :

L-255_10 mg

Product Name :

Lemborexant

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Lemberoxant (E2006) is a potent, brain-penetrant dual orexin 1/orexin 2 receptor antagonist. Lemberoxant binds to both orexin receptors and functionally inhibits them in a competitive manner with low nanomolar potency1.Orexins are highly conserved proteins known to control feeding behavior, sleep-wake rhythm, reward and addiction and energy balance. In addition, several reports show that orexin/receptor pathways are involved in pathological processes of neurological diseases such as narcolepsy, depression, ischemic stroke, drug addiction and Alzheimer's disease2.

Supplier :

Alomone Labs

Target :

OX1, OX2 orexin receptors

Long Description :

Lemborexant (#L-255) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Potent Antagonist of Orexin1/2 Receptors

MW :

410.43

Synonyms :

E-2006, (1R,2S)-2-{[(2,4-dimethylpyrimidin-5-yl)oxy]methyl}-2-(3-fluorophenyl)-N-(5-fluoropyridin-2-yl)cyclopropanecarboxamide)

Source :

Synthetic

Molecular formula :

C22H20F2N4O2

Effective Concentration :

10-500 nM

Activity :

Lemborexant is a potent and selective dual orexin receptor antagonist (IC50 of 60nM and 11nM on orexin 1 and 2 receptors, respectively). It is brain penetrant and orally bioavailable, demonstrating anxiolytic-like properties in rodents without affecting cognition and motor function1.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity

Solubility :

Soluble up to 50 mM in DMSO. Spin down all products before handling.

Storage of solutions :

Store at -20°C for several months. It is recommended to aliquot stock solutions to avoid repeated thawing and freezing.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99%

CAS No :

1369764-02-2

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(1R,2S)-2-[(2,4-dimethylpyrimidin-5-yl)oxymethyl]-2-(3-fluorophenyl)-N-(5-fluoropyridin-2-yl)cyclopropane-1-carboxamide

more info or order :

Alomone Labs product webpage