LASSBio-1135 | Alomone Labs L-190 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

LASSBio-1135

catalog :

L-190

more info or order :

Alomone Labs product webpage

image

image 1 :

image 2 :

Alomone Labs LASSBio-1135inhibits TRPV1 channels expressed in C6 cells. - mTRPV1-C6 cells were loaded with Fluo-3 AM. Changes in intracellular Ca2+were detected via changes in Fluo-3 emission following agonist application. 5 minutes pre-incubation with 10 MLASSBio-1135(#L-190) inhibited 10 MCapsaicin(#C-125)-evoked rise in intracellular Ca2+. Normalized fluorescence before and after application (arrow) of 10 M Capsaicin in cells pre-incubated with none (black) or 10 M LASSBio-1135 (gray).

product information

CAT :

L-190

SKU :

L-190_10 mg

Product Name :

LASSBio-1135

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Transient receptor potential (TRP) channels are important mediators of sensory signaling with significant effect on signal transduction pathways and cellular activity. TRPV is a member of the Vanilloid subfamily of TRP channels1.LASSBio-1135, also known as CHEMBL466188, is a synthetic antagonist of TRPV1 channels and a blocker of TNF-α channels. It has an effective concentration of 0.1-100 μM and an IC50 of 588 and 642 nM for TRPV1 and TNF-α receptors respectively. LASSbio belongs to a family of imidazo[1,2-a] pyridine derivatives and was found to be an inhibitor of p38 MAPK and COX2 in vivo. However, similar results have not been shown to this day in vitro where LASSbio failed to inhibit p38 MAPK directly and only slightly inhibited COX2. LASSbio is highly effective in completely reducing hyperalgesia induced by capsaicin and inhibits capsaicin evoked currents in a concentration dependent manner. LASSbio also inhibits TNF-α production in macrophages stimulated by LPS by reducing p38 MAPK activation through a modulation of an upstream signaling pathway thus making it a possible therapeutic agent for the prevention of neuropathic and other pain2.

Supplier :

Alomone Labs

Target :

TRPV1 channels, TNF-α receptors

Long Description :

LASSBio-1135 (#L-190) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Blocker of TRPV1 Channels and Antagonist of TNF-α Receptors

MW :

286.3

Synonyms :

CHEMBL466188

Source :

Synthetic

Molecular formula :

C18H14N4.

Effective Concentration :

0.1-100 µM.

Activity :

LASSBio-1135 is a TRPV1 channel and a TNF-α receptor antagonist1.

Reconstitution and Solubility :

25 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

25 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99%

CAS No :

852453-71-5

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

N-phenyl-2-pyridin-2-ylimidazo[1,2-a]pyridin-3-amine.

more info or order :

Alomone Labs product webpage