L-Ascorbic acid | Alomone Labs L-140 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

L-Ascorbic acid

catalog :

L-140

more info or order :

Alomone Labs product webpage

image

image 1 :

Expression of PMCA4 in rat parietal cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-PMCA4Antibody (#ACP-004) (1:300) followed by goat anti-rabbit-AlexaFluor-488 antibody. PMCA4 staining (green) appears in neuronal profiles in deep layers of the parietal cortex (arrows). Nuclei are labeled with DAPI (blue).

image 2 :

Alomone Labs L-Ascorbic acid inhibits T-type voltage-gated Ca2+currents expressed inXenopusoocytes. - A. Time course of CaV3.2/?3 (T-type) current inhibition by 5 M ofL-Ascorbic acid(#L-140). Currents were elicited by application of voltage ramps from a holding potential of -100 mV to 20 mV (100 msec) delivered every 10 seconds. B. Superimposed example traces of current responses before and during perfusion of 5 M L-Ascorbic acid as indicated.

product information

CAT :

L-140

SKU :

L-140_10 g

Product Name :

L-Ascorbic acid

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

The T-type calcium channel belongs to the voltage-gated Ca2+ channel (CaV) family. "T" stands for transient, referring to the length of activation. As with other sub-types of voltage-gated Ca2+ channels, the α1 subunit is the one that determines most of the channel's properties. T-type calcium channels may contain one of three α1 subunits, α1G (CaV3.1), α1H (CaV3.2) or α1I (CaV3.3)1-3.T-type Ca2+ channels are involved in the control of neuronal excitability and their gating can be modulated by a variety of redox agents4.L-Ascorbic acid (ascorbate) is an endogenous redox agent that selectively inhibits native CaV3.2 channels in the peripheral and central neurons4.L-Ascorbic acid has an IC50 of 6.5 µM on T-type current of rat DRG neurons, and IC50s of 10 and 25 nM on cloned CaV3.2 channels from human and rat, respectively4.

Supplier :

Alomone Labs

Target :

T-type Ca2+ channels

Long Description :

L-Ascorbic acid (#L-140) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Blocker of T-Type CaV Channels

MW :

176.1

Synonyms :

Vitamin C

Source :

Synthetic

Molecular formula :

C6H8O6.

Effective Concentration :

10 nM - 5 µM.

Activity :

IC50 of 6.5 µM on T-type current of rat DRG neurons1. IC50s of 10 and 25 nM on cloned CaV3.2 channels from human and rat, respectively1.

Reconstitution and Solubility :

Water. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

Water. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

50-81-7

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

3-Oxo-L-gulofuranolactone.

more info or order :

Alomone Labs product webpage