(-)-Lobeline hydrochloride | Alomone Labs L-120 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

(-)-Lobeline hydrochloride

catalog :

L-120

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs (-)-Lobeline hydrochloride inhibits the muscle?1/?1/?/?nAChR currents expressed inXenopusoocytes. - A. Time course of current reversible inhibition by increasing concentrations of(-)-Lobeline hydrochloride(#L-120). Currents were elicited by application of 100 M Acetylcholine every 100 seconds from a holding potential of -80 mV B. Superimposed example traces of current response to acetylcholine before (Cont) and during 1 5 and 10 M (-)-Lobeline hydrochloride application.

product information

CAT :

L-120

SKU :

L-120_1 g

Product Name :

(-)-Lobeline hydrochloride

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Nicotinic acetylcholine receptors (nAChRs) are important neuromodulatory cation channels which short-circuit their assimilating membrane by their primary endogenous agonist acetylcholine (ACh)1. nAChRs are highly expressed in myocytes and neurons and are comprised of at least 17 studied subunits, that can be classified into two types: α (α1 - α10) and non-α (β1-4, γ, δ and ε). Various nAChR subunits co-assemble to create muscular (α1β1γδ and α1β1εδ) and neuronal channels which are found in different and more restrictive combinations (α2-6 and β2-4 possible arrangements, e.g. α4β2)2,3.Lobeline hydrochloride is a lipophylic alkaloid which either agonizes or antagonizes the nicotine-evoked response of nAChRs. Lobeline acts as an anxiolytic, a cognition enhancer and a neurotransmitter-release stimulant in the CNS, whereas it inhibits the response to ACh at the α7 subunit with an IC50 of 8.5 μM, and decreases the endplate potential at the neuromuscular junction - thus attenuates locomotor sensitization4-7. Moreover, its high affinity (Ki = 4-20 nM) to the α4β2, nAChR subtype, along with a nonselective binding to monoamine transporters, renders it capable of preventing the psychostimulatory effects of drugs of abuse8.

Supplier :

Alomone Labs

Target :

nAChR

Long Description :

(-)-Lobeline hydrochloride (#L-120) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Potent nAChR Antagonist

MW :

373.9

Source :

Synthetic

Molecular formula :

C22H28ClNO2.

Effective Concentration :

0.1-100 µM.

Activity :

(-)-Lobeline hydrochloride is a high affinity modulator of nicotinic acetylcholine receptors. It inhibits α7 nAChRs with IC50 of 8.5 µM1.It also acts on the α4/β2 combination2.

Reconstitution and Solubility :

Ethanol/water. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

Ethanol/water. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

134-63-4

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

2-[(2R,6S)-6-[(2S)-2-Hydroxyphenethyl)]-1-methyl-2-piperidinyl]acetophenone hydrochloride.

more info or order :

Alomone Labs product webpage