KT5823 | Alomone Labs K-250 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

KT5823

catalog :

K-250

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Santos A, Carreira B, Nobre R, Carvalho C, Araújo I. Stimulation of neural stem cell proliferation by inhibition of phosphodiesterase 5. Stem Cells Int. 2014;2014:878397 pubmed publisher
Alonso Magdalena P, Laribi O, Ropero A, Fuentes E, Ripoll C, Soria B, et al. Low doses of bisphenol A and diethylstilbestrol impair Ca2+ signals in pancreatic alpha-cells through a nonclassical membrane estrogen receptor within intact islets of Langerhans. Environ Health Perspect. 2005;113:969-77 pubmed

image

image 1 :

Alomone Labs KT5823 inhibits PLC?3 phosphorylation via PKG in C6 glioma cells. - Cells were grown to 70% confluence and then serum starved for 18 h. The cells were then preincubated for 30 min in different concentrations ofKT5823(#K-250) and stimulated with 8Br-cGMP (100 M 4h 37C) for PKG activation. Cell proteins were resolved by SDS-PAGE and probed with anti phospho-PLC?3. PLC?3 is a substrate of PKG.

image 2 :

Peptide CDRILNKEGGIVPFKTK corresponding to amino acid residues 601-617 of rat PMCA4 (AccessionQ64542). 2ndintracellular loop.

image 3 :

product information

CAT :

K-250

SKU :

K-250_0.1 mg

Product Name :

KT5823

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

KT5823 is a semi-synthetic derivative of K525a.1 It is a potent, selective inhibitor of cGMP-dependent protein kinase (PKG) (in vitro IC50= 234 nM). KT5823 interferes at the level of the ATP binding site of the catalytic domain of PKG.2,3 The PKG are Ser/Thr protein kinase with broad substrate specificity, and expressed in; brain, smooth muscle cells, platelets, juxtaglomerular kidney cells, and endothelial cells.4,5 KT5823 triggered apoptotic DNA fragmentation in immortalized uterine epithelial cells, suggesting a regulatory role for PKG in the apoptotic process in these cells.6

Supplier :

Alomone Labs

Target :

L-Type CaV Channels

Long Description :

KT5823 (#K-250) is a highly pure, semi-synthetic, and biologically active compound.

Short Description :

A Selective PKG Inhibitor

MW :

495

Source :

Semi-synthetic

Molecular formula :

C29H25N3O5.

Effective Concentration :

500 nM - 1 µM.

Activity :

KT5823 is a selective and potent inhibitor of PKG (IC50 = 234 nM)1,2.

Reconstitution and Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to one week at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

126643-37-6

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(9S,10R,12R)-2,3,9,10,11,12-Hexahydro-10-methoxy-2,9-di methyl-1-oxo-9,12-epoxy-1H-diindolo[1,2,3-fg:3',2',1'-k l]pyrrolo[3,4-i][1,6]benzodiazocine-10-carboxylic acid, methyl ester.

more info or order :

Alomone Labs product webpage