KT5720 | Alomone Labs K-190 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

KT5720

catalog :

K-190

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Brackenbury W, Djamgoz M. Nerve growth factor enhances voltage-gated Na+ channel activity and Transwell migration in Mat-LyLu rat prostate cancer cell line. J Cell Physiol. 2007;210:602-8 pubmed

image

image 1 :

Alomone Labs KT5720 inhibits NGF-mediated survival of serum-deprived PC12 cells. - Dose-response curve of KT5720 (#K-190) inhibition ofNative mouse NGF 2.5S protein ( 95%) (#N-100) (50 ng/ml) mediated survival of serum-deprived PC12 cells. Cell viability was determined by XTT method and normalized to control. IC50was determined at 287.4 nM.

image 2 :

image 3 :

Alomone Labs KT5720 inhibits PKAS phosphorylation via PKA. - NIH/C6 cells were preincubated for 45 min with different concentrations ofKT5720(#K-190) and then stimulated with 10 MForskolin(#F-500) for 20 min. Cell proteins were resolved by SDS-PAGE and probed with antiphospho-(Ser/Thr) PKAS (PKA substrate).

product information

CAT :

K-190

SKU :

K-190_0.1 mg

Product Name :

KT5720

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

KT5720 is a semi-synthetic derivative of K525a. It is a cell-permeable, selective inhibitor of cAMP-dependent protein kinase (PKA; IC50 = 56 nM). No significant effect on protein kinase C (PKC), protein kinase G (PKG) or myosin light chain kinase (MLCK), was observed.1 KT5720 was used to examine the role of PKA in various cellular events such as cell adhesion,2 neurite branching,3 anterograde axonal transport,4 memory consolidation,5,6 and regulation of transcription by cyclic AMP-dependent response element-binding protein (CREB).7

Supplier :

Alomone Labs

Target :

PKA

Long Description :

KT5720 (#K-190) is a highly pure, semi-synthetic, and biologically active compound.

Short Description :

A Selective PKA Inhibitor

MW :

537

Source :

Semi-synthetic

Molecular formula :

C32H31N3O5.

Effective Concentration :

100-500 nM.

Activity :

KT5720 is a potent and specific inhibitor of PKA (IC50 = 56 nM)1.

Reconstitution and Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to one week at 4°C or three months at -20°C.Protect from light.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99% (HPLC)

CAS No :

108068-98-0

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(9R,10S,12S)-2,3,9,10,11,12-Hexahydro-10-hydroxy-9- meth yl-1-oxo-9,12-epoxy-1H-diindolo[1,2,3-fg:3',2',1'-kl]py rrolo[3,4- i][1,6]benzodiazocine-10-carboxylic acid, hexyl ester.

more info or order :

Alomone Labs product webpage