Ionomycin calcium salt | Alomone Labs I-700 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Ionomycin calcium salt

catalog :

I-700

more info or order :

Alomone Labs product webpage

citations: 7

Reference
Vu C, Fukushima S, Wazawa T, Nagai T. A highly-sensitive genetically encoded temperature indicator exploiting a temperature-responsive elastin-like polypeptide. Sci Rep. 2021;11:16519 pubmed publisher
Uzureau S, Lecordier L, Uzureau P, Hennig D, Graversen J, Homble F, et al. APOL1 C-Terminal Variants May Trigger Kidney Disease through Interference with APOL3 Control of Actomyosin. Cell Rep. 2020;30:3821-3836.e13 pubmed publisher
Vierra N, Dadi P, Milian S, Dickerson M, Jordan K, Gilon P, et al. TALK-1 channels control β cell endoplasmic reticulum Ca2+ homeostasis. Sci Signal. 2017;10: pubmed publisher
Wertheimer E, Krapf D, de la Vega Beltrán J, Sanchez Cardenas C, Navarrete F, Haddad D, et al. Compartmentalization of distinct cAMP signaling pathways in mammalian sperm. J Biol Chem. 2013;288:35307-20 pubmed publisher
Zamora R, Azhar N, Namas R, Metukuri M, Clermont T, Gladstone C, et al. Identification of a novel pathway of transforming growth factor-β1 regulation by extracellular NAD+ in mouse macrophages: in vitro and in silico studies. J Biol Chem. 2012;287:31003-14 pubmed publisher
Martínez López P, Trevino C, de la Vega Beltrán J, De Blas G, Monroy E, Beltrán C, et al. TRPM8 in mouse sperm detects temperature changes and may influence the acrosome reaction. J Cell Physiol. 2011;226:1620-31 pubmed publisher
De Blas G, Darszon A, Ocampo A, Serrano C, Castellano L, Hernández González E, et al. TRPM8, a versatile channel in human sperm. PLoS ONE. 2009;4:e6095 pubmed publisher

image

image 1 :

Alomone Labs Ionomycin calcium salt induces cytosolic Ca2+increase. - Ca2+traces from Fluo-3 AM loaded C6 glioma cells treated with 1 MIonomycin calcium salt(#I-700).

product information

CAT :

I-700

SKU :

I-700_1 mg

Product Name :

Ionomycin calcium salt

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Ionomycin is a narrow spectrum antibiotic for gram-positive bacteria produced by Streptomyces conglobatus. In mammalian cells, ionomycin acts as a potent and selective Ca2+ ionophore, more effective than A23187. The Ca2+ influx by ionomycin is caused by direct stimulation of store-regulated cation entry and not by direct action at the plasma membrane.1-4Ionomycin induces hydrolysis of phosphoinositides, activation of PKC in human T cells and apoptosis in various cells lines.5-7

Supplier :

Alomone Labs

Long Description :

Ionomycin calcium salt (#I-700) is a highly pure, natural, and biologically active compound.

Short Description :

A Potent and Selective Ca2+ Ionophore

MW :

747.08

Source :

Natural

Molecular formula :

C41H70O9Ca.

Effective Concentration :

100 nM - 2 µM.

Activity :

Ionomycin is a narrow spectrum antibiotic for gram-positive bacteria1,2. In mammalian cells, Ionomycin acts as a selective Ca2+ ionophore3. In addition, Ionomycin induces hydrolysis of phosphoinositides, activation of PKC in human T cells as well as apoptosis in various cell lines4-6.

Reconstitution and Solubility :

DMSO or methanol. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

DMSO or methanol. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to two week at 4°C or three months at -20°C.Protect from light.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99% (HPLC)

CAS No :

56092-82-1

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-11,19,21-Tri hydroxy-4,6,8,12,14,18,20-heptamethyl-22-[(2S,2'R,5S,5'S)-octahydro-5'-[(1R)-1-hydroxyethyl]-2,5'-dimethyl[2,2'-bifuran]-5-yl]-9-oxo-10,16-docosadienoic acid calcium salt.

more info or order :

Alomone Labs product webpage