Isopimaric Acid | Alomone Labs I-370 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Isopimaric Acid

catalog :

I-370

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Henney N, Li B, Elford C, Reviriego P, Campbell A, Wann K, et al. A large-conductance (BK) potassium channel subtype affects both growth and mineralization of human osteoblasts. Am J Physiol Cell Physiol. 2009;297:C1397-408 pubmed publisher
Lam F, Seto S, Kwan Y, Yeung J, Chan P. Activation of the iberiotoxin-sensitive BKCa channels by salvianolic acid B of the porcine coronary artery smooth muscle cells. Eur J Pharmacol. 2006;546:28-35 pubmed

image

image 1 :

Alomone Labs Isopimaric Acid was tested inXenopusoocytes expressing mSlo(BK) channel using TEVC recordings. - Time course of BK channel amplitudes by applying 100 ?MIsopimaric Acid(#I-370). Currents were elicited by a 150 ms pulse that was increased stepwise every 30 ms from +10 mV to +50 mV from a holding potential of -100 mV. Pulses were delivered every 10 sec. Pulses were normalized to the values of controls.

image 2 :

product information

CAT :

I-370

SKU :

I-370_1 mg

Product Name :

Isopimaric Acid

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Isopimaric acid (originally isolated from terpenoids) was found to be a potent opener of KCa1.1 (large conductance Ca2+-activated K+ (BK) channels. 1 µM Isopimaric acid doubled the current carried via KCa1.1 channels and this effect increased dose dependently. In analogy to the effects of pimaric acid, isopimaric acid may increase the open probability of single KCa1.1 channels by shifting both its voltage and Ca2+ dependence to more hyperpolarized voltages.1 KCa1.1 channels that were activated by isopimaric acid retain their charybdotoxin sensitivity.

Supplier :

Alomone Labs

Target :

KCa1.1 K+ channels

Long Description :

Isopimaric Acid (#I-370) is a highly pure, natural, and biologically active compound.

Short Description :

A Novel Opener of KCa1.1 Channels

MW :

302

Synonyms :

IPA

Source :

Natural

Molecular formula :

C20H30O2.

Effective Concentration :

1-100 μM.

Activity :

Isopimaric acid is a potent opener of large conductance Ca2+-activated K+ (BK) channels. 1 μM of Isopimaric acid doubles the current carried via BK channels. Isopimaric acid may increase the open probability by shifting the Ca2+ and voltage dependence to more hyperpolarized voltages1. The sensitivity is decreased in the Xenopus system compared to mammalian cells. BK channels activated by Isopimaric acid retain their charybdotoxin sensitivity.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.

Solubility :

DMSO, ethanol, acetone or methanol. DMSO is suggested for biological experiments. DMSO solution should not exceed 0.03%. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99%

CAS No :

5835-26-7

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

1-Phenanthrenecarboxylicacid,7-ethenyl- 1,2,3,4,4a,4b,5,6,7,8,10,10a-dodecahyde.

more info or order :

Alomone Labs product webpage