chemical

Isoguvacine hydrochloride

I-185

I-185_100 mg

Isoguvacine hydrochloride

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Isoguvacine hydrochloride is a specific and potent agonist of GABA(A) receptors with an EC50 value of 3.7-17.3 µM, depending on the subunit composition1. It is a semirigid analogue of trans-4-aminocrotonic acid with limited conformational flexibility and is less potent than GABA1,2.GABA(A) receptors are members of the Cys-loop superfamily of ligand-gated ion channels. They mediate GABA action, a major inhibitory neurotransmitter, through the central nervous system, and play an important role in inhibiting cell excitation in the central nervous system. GABA(A) receptors are considered to be a major target of antiseizure drugs3,4.

Alomone Labs

GABA(A) receptors

Isoguvacine hydrochloride (#I-185) is a highly pure, synthetic, and biologically active compound.

An Agonist of GABA(A) Receptors

163.6

Synthetic

C6H10ClNO2.

0.1-10 µM.

Isoguvacine hydrochloride is a specific agonist of the GABA(A) receptors, with EC50 of 3.7-17.3 µM, depending on subunit composition1.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.

Up to 100 mM in water. Centrifuge all product preparations before use (10000 x g, 5 min).

Up to four weeks at 4°C or three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>99%

68547-97-7

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

1,2,3,6-tetrahydropyridine-4-carboxylic acid;hydrochloride.