chemical

IDRA 21

I-140

I-140_10 mg

IDRA 21

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

IDRA 21 is a well characterized benzothiadiazine positive allosteric modulator of glutamate AMPA receptors. IDRA 21 produced a concentration-dependent inhibition of glutamate-induced inactivation of membrane currents in recombinant HEK 293 (human embryonic kidney) cells stably transfected with human GluR1/2 flip receptors. IDRA 21 doubled the charge transfer at a concentration of 70 μM, suggesting that this compound can facilitate excitatory neurotransmission via GluR 1/2 receptors. The drug originally considered in the nootropic class of agents (which have had limited success as cognitive-enhancing agents clinically) has been shown to inhibit the rate of desensitization of the AMPA receptor during prolonged exposure to competitive agonists. Thus, the drug acts to strengthen glutamatergic synapses and requires the release of endogenous glutamate for its pharmacological actions. This property of IDRA 21 supports its ability to enhance long-term potentiation (LTP), particularly within the hippocampus1.

Alomone Labs

AMPA receptors

IDRA 21 (#I-140) is a highly pure, synthetic, and biologically active compound.

A Positive Allosteric Modulator of AMPA Receptors

232.7

Synthetic

C8H9ClN2O2S.

10-500 µM.

IDRA 21 is a selective AMPA receptor agonist1.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

100 mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).

Up to four weeks at 4°C or three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>99%

22503-72-6

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

7-Chloro-3-methyl-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide.