ICA-27243 | Alomone Labs I-130 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

ICA-27243

catalog :

I-130

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Berg T. Kv7(KCNQ)-K+-Channels Influence Total Peripheral Resistance in Female but Not Male Rats, and Hamper Catecholamine Release in Hypertensive Rats of Both Sexes. Front Physiol. 2018;9:117 pubmed publisher

image

image 1 :

Cell surface detection of GPR68 inlive intact human THP-1 monocytic leukemia cells: - ___ Cells. ___ Cells + goat-anti-rabbit-FITC. ___ Cells + Anti-GPR68 (OGR1) (extracellular) Antibody(#AGR-042) (2.5 g) + goat-anti-rabbit-FITC.

image 2 :

Alomone Labs ICA-27243 modulate KCNQ2/KCNQ3voltage-gated K+ currents expressed inXenopusoocytes. - A. Time course of KCNQ2/KCNQ3current enhancement by 2 and 10 MICA-27243(#I-130) as measured at -25 mV. Currents were elicited by application of voltage ramp from a holding potential of -100 mV to 0 mV (800 msec).B. Superimposed example traces of current responses before and during perfusion of 10 M ICA-27243 as indicated.

product information

CAT :

I-130

SKU :

I-130_10 mg

Product Name :

ICA-27243

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

KCNQ2 (KV7.2) and KCNQ3 (KV7.3) are voltage-gated K+ channel subunits that underlie the neuronal M current. ICA-27243 is a novel, potent and selective activator of M-current and KCNQ2/Q3 channels. ICA-27243 is structurally distinct from other known KCNQ/M-current activators. It enhances KCNQ channel activation by shifting channel opening to more hyperpolarized potentials. ICA-27243 activates KCNQ currents in Chinese hamster ovary cells expressing recombinant KCNQ2/Q3 heteromultimeric channels with an EC50 of 0.4 μM. ICA-27243 exhibits selectivity for KCNQ2/Q3 over KCNQ4 (20-fold) and KCNQ3/Q5 (>100-fold) and has no effect on GABA-activated chloride channels, neuronal Na+ channels, or voltage-gated Ca2+ channels. In addition, it produces minimal displacement of radioligand binding to a range of central nervous system receptors and transporters. ICA-27243 also suppresses seizure-like activity in an ex vivo hippocampal slice model, and protects against maximal electroshock-induced seizures in mice1.

Supplier :

Alomone Labs

Target :

KCNQ2, KCNQ3 K+ channels

Long Description :

ICA-27243 (#I-130) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Potent and Selective Opener of KCNQ2 and KCNQ3 K+ Channels

MW :

268.64

Source :

Synthetic

Molecular formula :

C12H7ClF2N2O.

Effective Concentration :

0.5-50 µM.

Activity :

ICA-27243 is a potent and selective KCNQ channel modulator (enhancer)1 with EC50 ~ 1 µM.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. Soluble in water. Once dissolved in water, add an equal volume of glycerol. Do not shake or vortex. Avoid multiple freeze-thawing cycles

Solubility :

100 mM DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99%

CAS No :

325457-89-4

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

N-(6-Chloro-pyridin-3-yl)-3,4-difluoro-benzamide.

more info or order :

Alomone Labs product webpage