HA-1077 | Alomone Labs H-220 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

HA-1077

catalog :

H-220

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs TCN 201 inhibits NMDA receptor channels expressed inXenopusoocytes. - A. Time course of NR1/NR2A currents elicited by transient stimulations with 100 M glutamate and 100 M glycine every 100 sec while membrane potential was held at -80 mV. Application of 1 MTCN 201(#T-190) for 5 min (green) significantly inhibits current amplitude. B. Superimposed current traces from the recording shown in A upon application of control (black) and of 1 M TCN 201 (green).

image 2 :

image 3 :

Alomone Labs HA-1077 inhibits the phosphorylation of myosin light chain (MLC) in C2C12 cells. - Cells were grown in fetal calf serum (FCS) to 2 days post-confluence. They were then stimulated with 2% horse serum (HS) or not (FCS) in the presence or absence of 20 MHA-1077(#H-220) for the indicated times. Cell proteins were resolved by SDS-PAGE and probed with anti-phospho- MLC.

product information

CAT :

H-220

SKU :

H-220_10 mg

Product Name :

HA-1077

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

HA-1077 is a small, cell permeable derivative of isoquinoline which specifically inhibits the small GTPase - Rho by inhibition of its associated kinase-Rho dependent kinase (ROK). This signaling pathway acts as a molecular switch to control cytoskeleton organization in many cell types including smooth muscle.1-3 By inhibition of ROK, HA-1077 decreases the myosin light chain (MLC) phosphorylation induced by Ca2+ due to abrogating Rho-mediated inhibition of MLC dephosphorylation. HA-1077 also inhibits fetal calf serum-induced proliferation and [3H]thymidine incorporation into DNA of growth-arrested VSMC in a dose-dependent manner.4-6At concentrations higher than 5 µM, it induces disorganization of actin filament structure. This compound was also found to act as a vasodilator and antivasospasm drug which antagonizes the verapamil-insensitive voltage dependent Ca2+ channel and is used clinically for treatment of the cerebral vasospasm after subarachnoid hemorrhage.7-9

Supplier :

Alomone Labs

Target :

Rho/Rok

Long Description :

HA-1077 (#H-220) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Rho/Rok Inhibitor

MW :

364.3

Synonyms :

Fasudil, Eril, AT-877

Source :

Synthetic

Molecular formula :

C14H17N3O2S•2HCl.

Effective Concentration :

>1 µM.

Activity :

HA-1077 specifically inhibits the small GTPase - Rho through its associated kinase-Rho dependent kinase (ROK), thereby inhibiting myosin llight chain (MLC) phosphorylation1-3. It also acts as an intracellular Ca2+ antagonist4.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.

Solubility :

Water, DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to two weeks at 4°C or six months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99%

CAS No :

103745-39-7

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

1-(5-Isoquinolinesulfonyl) homopiperazine, dihydrochloride salt.

more info or order :

Alomone Labs product webpage