chemical

HMA

H-130

H-130_100 mg

HMA

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

HMA is a TRPA1 channel blocker with an IC50 value of 35 µM, an inhibitor of the Na+/H+ exchangers (NHE) and a blocker of ASIC3 channel (acid-sensing ion channels)1-3. HMA also antagonizes P2X7 receptors, although it is not potent4.HMA as an NHE inhibitor, is considered to be a promising adjuvant drug for overcoming acquired resistance to treatment in various malignant hemopathies1-3.

Alomone Labs

NHE exchangers, TRPA1, ASIC3 channels, P2X7 receptors

HMA (#H-130) is a highly pure, synthetic, and biologically active compound.

An Antagonist of Na+/H+ Exchangers, P2X7 receptors and TRPA1, ASIC3 Channels

311.77

5-(N,N-Hexamethylene) amiloride

Synthetic

C12H18ClN7O.

1-300 µM.

HMA is an amiloride derivative, shown to inhibit TRPA1-mediated calcium signal with IC50 of 35 µM1. It is an inhibitor of the Na+/H+ exchanger (NHE), with Ki of 0.013-2.4 µM for various NHE isoforms2. HMA also blocks ASIC3 channels by 51% at 20 µM3.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.

Up to 100mM in DMSO. Centrifuge all products before use (10000 x g 5 min).

Up to three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>98%

1428-95-1

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

3-amino-5-(azepan-1-yl)-6-chloro-N-(diaminomethylidene)pyrazine-2-carboxamide.