Neurotensin | Alomone Labs GPN-100 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Neurotensin

catalog :

GPN-100

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Tauris J, Gustafsen C, Christensen E, Jansen P, Nykjaer A, Nyengaard J, et al. Proneurotrophin-3 may induce Sortilin-dependent death in inner ear neurons. Eur J Neurosci. 2011;33:622-31 pubmed publisher

image

image 1 :

Peptide (C)VLEKVPVSKQLK corresponding to amino acid residues 166-178 of human BDNF (precursor) (AccessionP23560).

image 2 :

Alomone Labs Neurotensin induces Ca2+influx in HT29 cells. - Cells were loaded with Fluo-3AM Ca2+probe. Ca2+influx was measured in the presence of increasingNeurotensin(#GPN-100) concentrations. The maximum Ca2+levels is plotted for each neurotensin concentration (ED50= 142 ng/ml).

product information

cat :

GPN-100

SKU :

GPN-100_1 mg

Product Name :

Neurotensin

Group Type :

Non Antibodies

Product Type :

Proteins

Applications :

Calcium imaging assay

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Source :

Synthetic peptide

Gene ID :

NTSR1, NTSR2

Product Page - Scientific background :

Neurotensin is a peptide neurotransmitter hormone produced from a 170 amino acid precursor protein, first isolated in 1973 from bovine hypothalamus1. Neurotensin signaling is transmitted through three receptors, NTS1 and NTS2 are 7-transmembrane G-protein coupled receptors, and NTS3 is a type I membrane receptor with a large extra cellular domain2.Neurotensin synaptic transmission is terminated primarily by specific enzymatic cleavage of neurotensin by several peptidases3. Neurotensin participates in the regulation of Hypophyseal Prolactin and Luteinizing Hormone (LH) secretion4 and has a pivotal role in the dopaminergic system regulation.Neurotensin is implicated in body temperature regulation, feeding behavior, locomotion regulation5 and pain transmission6.GPCR ligands are now part of our growing portfolio of GPCR related products, which also includes antibodies.

Supplier :

Alomone Labs

Target :

Neurotensin Receptors

Long Description :

A Ligand of Neurotensin G-Protein Coupled Receptor

Short Description :

A Ligand of Neurotensin G-Protein Coupled Receptor

MW :

1673 Da

Synonyms :

NTS, NTS1, NT

Modifications :

Glu1 - Pyrrolidone carboxylic acid

Molecular formula :

C78H121N21O20

Effective Concentration :

5 - 200 nM

Activity :

Neurotensin is an endocrinic or paracrinic hormone. It plays a role in the regulation of fat metabolism, feeding suppression, circadian pacemaker, anti-psychotic-like action, body temperature, dopamine neurotransmission, and smooth muscle contraction and vasodilatation1,2.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

CAS No :

55508-42-4

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

ELYENKPRRPYIL-OH

Is Toxin :

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage