chemical

N-Formyl-Met-Leu-Phe

GPF-100

GPF-100_10 mg

N-Formyl-Met-Leu-Phe

Non Antibodies

Proteins

Calcium imaging assay

Lyophilized Powder.

Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. Soluble in DMSO. Prepare a concentrated stock solution by dissolving the lyophilized peptide in DMSO first (e.g., at a concentration between 100-1000x of the final working concentration). Once the peptide is completely dissolved in DMSO, slowly dilute the solution into the working buffer (or water) to the desired final working concentration. Centrifuge all product preparations before use. Avoid multiple freeze-thaw cycles to maintain biological activity. It is recommended to keep the DMSO concentration as low as possible. For cell assays, a final concentration of 0.1%–0.5% DMSO (v/v) is considered safe. For other experiments, a 5% DMSO (v/v) concentration is recommended.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes). The lyophilizate may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. Soluble in DMSO. Prepare a concentrated stock solution by dissolving the lyophilized peptide in DMSO first (e.g., at a concentration between 100-1000x of the final working concentration). Once the peptide is completely dissolved in DMSO, slowly dilute the solution into the working buffer (or water) to the desired final working concentration. Centrifuge all product preparations before use. It is recommended to keep the DMSO concentration as low as possible. For cell assays, a final concentration of 0.1%–0.5% DMSO (v/v) is considered safe. For other experiments, a 5% DMSO (v/v) concentration is recommended.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Synthetic peptide

FPR1

Chemotactic factors from both Gram-positive and Gram-negative bacteria are short peptides with N-formyl methionine at the N-terminus (extensively reviewed in reference 1). These peptides are released from bacteria during infection and activate formyl peptide receptors (FPR), members of the G-protein coupled receptor (GPCR) superfamily. In humans, the FPR family consists mainly of three receptors, FPR1, FPR2/ALX (formerly FPRL1), and FPR3 (formerly FPRL2) which all couple to the Gi subtype of G-proteins and ultimately lead to the activation of phospholipase C and intracellular Ca2+ increase1,2.N-Formyl-Met-Leu-Phe is a selective and potent agonist of the Formyl peptide receptor (FPR1)3.In human polymorphonuclear leukocytes N-formyl-met-leu-phe activates p38 by a process involving phosphatidylinositol 3-kinase, protein kinase C, and calcium4. N-Formyl-Met-Leu-Phe increased in a dose-dependent manner (0.1 nM - 1 µM) the adherence of neutrophils to vascular endothelial cells which is the initial event in the migration of neutrophils through blood vessel walls to tissue sites of inflammation5.

Alomone Labs

FPR1 receptor

A Potent Agonist of FPR1 G-Protein Coupled Receptor

A Potent Agonist of FPR1 G-Protein Coupled Receptor

437.6 Da

FMLP, N-formyl-L-methionyl-L-leucyl-L-phenylalanine, N-formyl-MLF

Met1 - N-formyl-Met.

C21H31N3O5S

1 nM - 10 μM

N-Formyl-Met-Leu-Phe is a selective and potent agonist of the formyl peptide receptor (FPR1)1.

Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day.

1-2 Business Days

Israel/IL

≥98% (HPLC)

59880-97-6

Lyophilized

Contact Alomone Labs for technical support and product customization

MLF-OH

No

12352202

yes

no