Contulakin-G | Alomone Labs GPC-100 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Contulakin-G

catalog :

GPC-100

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs Contulakin-G increases intracellular Ca2+concentration in HT-29 cells. - Cells were loaded with Fluo-3 AM. Changes in intracellular Ca2+were detected via changes in Fluo-3 emission followingContulakin-G(#GPC-100) application. A. Normalized fluorescence before and after application (at 20 seconds see arrow) of 0-3.3 ?M Contulakin-G (as indicated). B. Normalized fluorescence plotted against Contulakin-G concentrations (n = 2 standard deviation indicated by error bars).

product information

cat :

GPC-100

SKU :

GPC-100_0.1 mg

Product Name :

Contulakin-G

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

Q9XYR5

Accession Number :

https://www.uniprot.org/uniprotkb/Q9XYR5/entry

Applications :

Calcium imaging assay

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus geographus (Geography cone) (Nubecula geographus)

Source :

Synthetic peptide

Gene ID :

NTSR1, NTSR2, SORT1

Product Page - Scientific background :

Contulakin-G acts as an agonist of neurotensin receptors. It binds to human neurotensin type 1 receptor (NTSR1), rat neurotensin types 1 and 2 receptors (NTSR1/NTSR2) and mouse neurotensin type 3 receptor (SORT1)1.Contulakin-G is a peptide initially isolated and purified from the marine snail Conus geographus venom based on its ability to induce sluggishness in mice2.O-linked glycosylation at Threonine residue at position 10 appears to be a highly unusual strategy for increasing the efficacy of toxins directed against neurotransmitter receptors1. Although the binding affinity of Contulakin-G for cloned neurotensin receptors in vitro proved to be significantly lower than neurotensin, Contulakin-G was shown to be a neurotensin receptor agonist at physiologically relevant concentrations1,2.

Supplier :

Alomone Labs

Target :

Neurotensin receptors

Long Description :

A Neurotensin Receptor Ligand

Short Description :

A Neurotensin Receptor Ligand

MW :

1704.8 Da

Synonyms :

CGX-1160

Modifications :

Z = Pyrrolidone carboxylic acid

Molecular formula :

C74H117N19O27

Effective Concentration :

100 nM - 1 µM

Activity :

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

CAS No :

229180-41-0

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

ZSEEGGSNATKKPYIL-OH

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage