Angiotensin III | Alomone Labs GPA-130 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Angiotensin III

catalog :

GPA-130

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Farmer G, Amune A, Bachelor M, Duong P, Yuan J, Cunningham J. Sniffer cells for the detection of neural Angiotensin II in vitro. Sci Rep. 2019;9:8820 pubmed publisher

image

image 1 :

Alomone Labs CNS-1102 inhibits NMDA receptor channels expressed inXenopusoocytes. - A. Time course of NR1/NR2A currents elicited by transient stimulations with 100 M glutamate and 100 M glycine every 100s while membrane potential was held at -80 mV. Application of 200 nMCNS-1102(#C-305) for 10 min (green) significantly inhibits current amplitude. B. Superimposed current traces from the recording shown in A upon application of control (black) and of 200 nM CNS-1102 (green).

image 2 :

Alomone Labs Angiotensin III induces MAPK activation in IEC-6 cells similar to Angiotensin II. - Cells were incubated in serum depleted medium for 2 h and then stimulated with 0.1 M and 1 MAngiotensin III(#GPA-130) for 10 min or with 0.1 M and 1 MAngiotensin II(#GPA-100) for comparison. Cell proteins were resolved by SDS-PAGE and probed with anti-phospo-ERK1/2.

product information

cat :

GPA-130

SKU :

GPA-130_10 mg

Product Name :

Angiotensin III

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P01019

Accession Number :

https://www.uniprot.org/uniprotkb/P01019/entry

Applications :

Western blot

Formulation :

Lyophilized Powder.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Source :

Synthetic peptide

Gene ID :

AGTR1, AGTR2

Product Page - Scientific background :

Angiotensin III (Ang III) is a hexapeptide formed from cleavage at the N-terminus of Angiotensin II (Ang II)1, a key factor in the Renin-Angiotensin-Aldosterone (RAAS) systeM In peripheral Ang systems, Ang II is the main effector peptide in the systemic circulation, although exogenous Ang III can be as potent as Ang II in, for example, stimulating aldosterone secretion2 or inhibiting renin release3. In the rat brain, Ang III was found to be equipotent with Ang II as a pressor agent or dipsogen4-7 and was bound as avidly to the nervous system as Ang II. Angiotensin receptor subtype AT1 has greater affinity towards Ang II and is also responsive to Ang III, while the AT2 receptor subtype appears to be more sensitive to Ang III but less responsive to Ang II1.

Supplier :

Alomone Labs

Target :

Angiotensin II receptors

Long Description :

A Ligand of AT1 and AT2 G-Protein Coupled Receptors

Short Description :

A Ligand of AT1 and AT2 G-Protein Coupled Receptors

MW :

931.1 Da

Synonyms :

Ang III, Angiotensin-3, des-As1-angiotensin II

Molecular formula :

C46H66N12O9

Effective Concentration :

1 nM - 1 μM

Activity :

Angiotensin III is an endogenous ligand of the AT1 and AT2 receptors and like Angiotensin II, regulates blood vessel tonus, blood pressure, and salt and water balance1-2.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

CAS No :

13602-53-4

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

RVYIHPF-OH

Is Toxin :

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage