Angiotensin II | Alomone Labs GPA-100 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Angiotensin II

catalog :

GPA-100

more info or order :

Alomone Labs product webpage

citations: 6

Reference
Raupp D, Fernandes R, Antunes K, Perin F, Rigatto K. Impact of angiotensin II type 1 and G-protein-coupled Mas receptor expression on the pulmonary performance of patients with idiopathic pulmonary fibrosis. Peptides. 2020;133:170384 pubmed publisher
Qi R, Li T, Chen X. The role of the renin-angiotensin system in regulating endometrial neovascularization during the peri-implantation period: literature review and preliminary data. Ther Adv Endocrinol Metab. 2020;11:2042018820920560 pubmed publisher
Kimura Y, Kuno A, Tanno M, Sato T, Ohno K, Shibata S, et al. Canagliflozin, a sodium-glucose cotransporter 2 inhibitor, normalizes renal susceptibility to type 1 cardiorenal syndrome through reduction of renal oxidative stress in diabetic rats. J Diabetes Investig. 2019;10:933-946 pubmed publisher
Gómez G, Fernandez P, Velarde V, Saez J. Angiotensin II-Induced Mesangial Cell Damaged Is Preceded by Cell Membrane Permeabilization Due to Upregulation of Non-Selective Channels. Int J Mol Sci. 2018;19: pubmed publisher
Yang T, Zhang H, Liang Q, Shi Y, Mei Y, Barrett P, et al. Small-Conductance Ca2+-Activated Potassium Channels Negatively Regulate Aldosterone Secretion in Human Adrenocortical Cells. Hypertension. 2016;68:785-95 pubmed publisher
Qi Y, Li H, Shenoy V, Li Q, Wong F, Zhang L, et al. Moderate cardiac-selective overexpression of angiotensin II type 2 receptor protects cardiac functions from ischaemic injury. Exp Physiol. 2012;97:89-101 pubmed publisher

image

image 1 :

Alomone Labs Angiotensin II induces MAPK activation in IEC-6 cells. - Cells were incubated in serum depleted medium for 2 h and then stimulated with 100 nMAngiotensin II(#GPA-100) for 5 or 10 min. Cell proteins were resolved by SDS-PAGE and probed with anti-phospo-ERK1/2.

product information

cat :

GPA-100

SKU :

GPA-100_1 mg

Product Name :

Angiotensin II

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P01019

Accession Number :

https://www.uniprot.org/uniprotkb/P01019/entry

Applications :

Western blot

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Source :

Synthetic peptide

Gene ID :

AGTR1, AGTR2

Product Page - Scientific background :

Angiotensin II (Ang II) is a key factor in the Renin-Angiotensin-Aldosterone (RAAS) systeM Ang II is a derivative of a precursor molecule - angiotensinogen, a member of the serpin family. Angiotensinogen is a substrate of the Renin in the kidney to produce a deca peptide - Ang I, which appears to have no biological activity. Ang I is converted to Ang II by Angiotensin-Converting Enzyme (ACE)1,2.The biological effects caused by Ang II are mediated by four Ang II receptor subtypes: AT1 and AT2. Ang II plays a critical role in the regulation of the renal and cardiovascular systems, and some activities in the central nervous systems. Circulating levels of Ang II were identified as controlling blood pressure, based on the regulation of salt and water metabolism, vascular smooth muscle cell tone, thirst, and sympathetic system outflow. Ang II is well known to cause potent increases in systemic and local blood pressure via its vasoconstrictive effect, to influence renal tubules, to retain Na+ and water by activating Na+/H+ exchangers, and by stimulating aldosterone release from the adrenal gland3.Ang II is also a cell growth modulator depending on the Ang II receptor subtype expressed in the cell membrane. It may either stimulate growth (proliferation, hypertrophy) or act as a growth suppressor3,4.

Supplier :

Alomone Labs

Target :

Angiotensin II receptors

Long Description :

A Ligand of AT1 and AT2 G-Protein Coupled Receptors

Short Description :

A Ligand of AT1 and AT2 G-Protein Coupled Receptors

MW :

1046.2 Da

Synonyms :

Ang II, Angiotensin-2

Molecular formula :

C50H71N13O12

Effective Concentration :

1 - 300 nM

Activity :

Angiotensin II regulates blood vessel tonus, blood pressure, and salt and water balance1-3.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

CAS No :

4474-91-3

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

DRVYIHPF-OH

Is Toxin :

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage