Genistein | Alomone Labs G-300 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Genistein

catalog :

G-300

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Grzelka K, Kurowski P, Gawlak M, Szulczyk P. Noradrenaline Modulates the Membrane Potential and Holding Current of Medial Prefrontal Cortex Pyramidal Neurons via ?1-Adrenergic Receptors and HCN Channels. Front Cell Neurosci. 2017;11:341 pubmed publisher

image

image 1 :

Alomone Labs Genistein inhibits tyrosine kinase in HeLa cells. - Cells were grown to 70% confluence and then starved for 18 h. The cells were preincubated for 30 min in the presence of 0.5 MGenistein(#G-300) and then stimulated for 2 h with 20% fetal calf serum or culture medium. The cell extracts were blotted and probed with phospho-Tyr specific antibodies.

image 2 :

Alomone Labs L-AP4 inhibits specific binding of L-Glutamic acid to rat brain glutamate receptors. - Percent inhibition of specific binding of 3.75 nM [3H]-L-Glutamic acid to membranes from whole rat brain (except cerebellum) was plotted against concentrations ofL-AP4(#L-150) (green circles) and of L-Glutamic acid (assay standard black squares) and used to calculate IC50and Ki values for L-AP4 (IC50? 4.49 M and Ki ? 4.43 M).

product information

CAT :

G-300

SKU :

G-300_10 mg

Product Name :

Genistein

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

An isoflavonoid derived from soy products,1 Genistein inhibits protein tyrosine kinases including the EGF receptor kinase (IC50 = 2.6 µM). The inhibition is competitive with respect to ATP and non-competitive with respect to the phosphate acceptor.1,2 It has a trivial effect on the activity of PKA and PKC (IC50 > 350 µM).1,2 In addition, Genistein inhibits Topoisomerase II activity,3 causes cell cycle arrest and apoptosis in Jurkat T-leukemia cells.4 It down regulates the transcription of genes involved in angiogenesis,5,6 and inhibits the action of GABA on recombinant GABA-A receptors.7 Genistein also inhibits tumor cell proliferation and induces tumor cell differentiation.8-10

Supplier :

Alomone Labs

Long Description :

Genistein (#G-300) is a highly pure, natural, and biologically active compound.

Short Description :

A Tyrosine Kinase Inhibitor

MW :

270.2

Source :

Natural

Molecular formula :

C15H10O5.

Effective Concentration :

0.5-10 µM.

Activity :

Genistein, apart from a broad range of biological roles, is well established as a tyrosine kinase inhibitor1.

Reconstitution and Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to one week at 4°C or three months at -20°C.Protect from light.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99%

CAS No :

446-72-0

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

5,7-Dihydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one.

more info or order :

Alomone Labs product webpage