Gabazine | Alomone Labs G-215 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Gabazine

catalog :

G-215

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Postnikova T, Griflyuk A, Amakhin D, Kovalenko A, Soboleva E, Zubareva O, et al. Early Life Febrile Seizures Impair Hippocampal Synaptic Plasticity in Young Rats. Int J Mol Sci. 2021;22: pubmed publisher
Turecek J, Regehr W. Cerebellar and vestibular nuclear synapses in the inferior olive have distinct release kinetics and neurotransmitters. elife. 2020;9: pubmed publisher
Chowdhury S, Matsubara T, Miyazaki T, Ono D, Fukatsu N, Abe M, et al. GABA neurons in the ventral tegmental area regulate non-rapid eye movement sleep in mice. elife. 2019;8: pubmed publisher
Govindaiah G, Kang Y, Lewis H, Chung L, Clement E, Greenfield L, et al. Group I metabotropic glutamate receptors generate two types of intrinsic membrane oscillations in hippocampal oriens/alveus interneurons. Neuropharmacology. 2018;139:150-162 pubmed publisher
Quattrocolo G, Maccaferri G. Novel GABAergic circuits mediating excitation/inhibition of Cajal-Retzius cells in the developing hippocampus. J Neurosci. 2013;33:5486-98 pubmed publisher

product information

CAT :

G-215

SKU :

G-215_10 mg

Product Name :

Gabazine

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Gabazine (SR-95531) is a specific, competitive and potent antagonist of GABA(A) receptors. The compound also blocks glycine receptors but with lower affinity. SR-95531 acts as an allosteric inhibitor of GABA(A) receptor channel opening1,2 and blocks GABA-evoked currents with an IC50 of 0.44 µM1. When SR 95531 was administered intravenously it was shown to cause seizures in mice3.GABA(A) receptors are members of the Cys-loop superfamily of ligand-gated ion channels. They mediate the GABA action, a major inhibitory neurotransmitter, throughout the central nervous system (CNS) and play an important role in inhibiting cell excitation in the CNS. The GABA(A) receptor is considered to be a major target of anti-seizure drugs4,5.

Supplier :

Alomone Labs

Target :

GABA(A), Glycine receptors

Long Description :

Gabazine (#G-215) is a highly pure, synthetic, and biologically active compound.

Short Description :

An Antagonist of GABA(A) and Glycine Receptors

MW :

287.32

Synonyms :

SR 95531

Source :

Synthetic

Molecular formula :

C15H17N3O3.

Effective Concentration :

0.1-10 µM.

Activity :

Gabazine is a competitive antagonist of the GABA(A) receptor, selectively inhibiting GABA-evoked currents (IC50 = 0.44 µM), but not currents evoked by pentobarbiton and etomidate1. It is also a low-affinity glycine receptor antagonist (KB = 191 µM)2.

Reconstitution and Solubility :

Soluble in methanol. Centrifuge all product preparations before use (10000 x g, 5 min).

Solubility :

Soluble in methanol. Centrifuge all product preparations before use (10000 x g, 5 min).

Storage of solutions :

Up to three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98% (HPLC)

CAS No :

105538-73-6

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Cited Application :

Electrophysiology

Chemical Name :

4-[6-imino-3-(4-methoxyphenyl)pyridazin-1-yl]butanoic acid.

more info or order :

Alomone Labs product webpage