chemical

γ-Aminobutyric acid

G-110

G-110_100 g

γ-Aminobutyric acid

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

γ-aminobutyric acid (GABA) is an amino acid derivate, synthesized from glutamate by the pyridoxal-L-phosphate (PLP)-dependent enzyme, glutamate decarboxylase (GAD)1. GABA provides the primary inhibitory signaling in the adult mammalian brain (CNS), and, to some degree of controversy, exerts a depolarizing response in the developing brain2.GABA is an agonist of two main receptor classes in the CNS: GABAA, an ionic ligand gated channel, and GABAB, a metabotrophic, G-protein coupled receptor. GABAA is assembled by 5 out of 16 possible subunits with α, β and either a γ or a δ (2:2:1 stoichiometry) being the most common. With an EC50 between 3 - 12μM it interacts with GABA to allow either an influx or an efflux of Cl- ions, into and out of the cell, respectively3. GABAB is a heterodimer comprised of the subunits GABAB1 and GABAB2. It reduces neurotransmitter release by inhibiting Ca2+ channels when activated presynaptically, and facilitates inward rectifying potassium channels, postsynaptically1.GABA deficiency is often the prime cause for epilepsy, with other notably disorders being autism4, spasticity, anxiety disorders, schizophrenia, and stiff-person syndrome5.

Alomone Labs

GABA(A), GABA(B) receptors, GABA transporters

γ-Aminobutyric acid (#G-110) is a highly pure, synthetic, and biologically active compound.

A Ligand of GABA(A), GABA(B) Receptors and GABA Transporters

103.1

GABA

Synthetic

C4H9NO2.

0.1-100 µM

1-100 µM activated recombinant α1β1γ2 channels with EC50 = 5 μM1.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.

Water. Centrifuge all product preparations before use (10000 x g 5 min).

Up to four weeks at 4°C or three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>98%

56-12-2

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

4-Aminobutanoic acid.