FK-506 | Alomone Labs F-900 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

FK-506

catalog :

F-900

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Zhou M, Kamarshi V, Arvin A, Oliver S. Calcineurin phosphatase activity regulates Varicella-Zoster Virus induced cell-cell fusion. PLoS Pathog. 2020;16:e1009022 pubmed publisher
ULRICH J, Kim M, Houlihan P, Shutov L, Mohapatra D, Strack S, et al. Distinct activation properties of the nuclear factor of activated T-cells (NFAT) isoforms NFATc3 and NFATc4 in neurons. J Biol Chem. 2012;287:37594-609 pubmed publisher
Kim M, Usachev Y. Mitochondrial Ca2+ cycling facilitates activation of the transcription factor NFAT in sensory neurons. J Neurosci. 2009;29:12101-14 pubmed publisher

image

image 1 :

Alomone Labs FK-506 inhibits the anti-adipogenic effect of calcineurin in 3T3-L1 cells. - Cells were induce to undergo differentiation with 1 g/ml insulin for 4 days (A B and C). The cells were treated for the first 4 days of differentiation with 1 MIonomycin(#I-700) (B and C) in the presence of 5 ng/ml (C) or absence (A and B) ofFK-506(#F-900). After 10 days the cells were stained with Oil red O and visualized with light microscopy.

image 2 :

Peptide (C)GPDDRRRWGSRGPR corresponding to amino acid residues 249-262 of mouse prostanoid EP1 receptor (AccessionP35375). 3rd extracellular loop.

image 3 :

product information

CAT :

F-900

SKU :

F-900_1 mg

Product Name :

FK-506

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

FK 506 (Tacrolimus), a macrolide compound isolated from Streptomyces tsukubaensis, is a potent immunosuppressive compound that inhibits T-cell activation and is an inhibitor of fungal growth (10 to 100 times more potent than Cyclosporin A).1-3 FK-506 binds and inhibits, in vitro, the activity of immunophilin proteins known as FK 506-binding protein (FKBP), in a variety of organisms, ranging from yeast to humans.4-8Immunosuppression activity of FK-506 seems to be due to FK-506/FKBP complex that inhibits the heterodimeric Ca2+/calmodulin-dependent phosphatase Calcineurin. This, in turn, prevents the Calcineurin-dependent activation of interleukin-2 transcription and T-cell proliferation.9-12 FK-506 inhibits FKBP activities, increases sarcoplasmic reticulum Ca2+ release and decreases the ability of Na+/Ca2+ exchange to extrude Ca2+ from the cell.13

Supplier :

Alomone Labs

Long Description :

FK-506 (#F-900) is a highly pure, natural, and biologically active compound.

Short Description :

Calcineurin (PP-2B) Inhibitor-Immunosuppressant

MW :

804.03

Synonyms :

Tacrolimus, Fujimycin

Source :

Natural

Molecular formula :

C44H69NO12.

Effective Concentration :

10 pM - 50 nM.

Activity :

FK-506 is an immunosuppressant and inhibits calcineurin (PP-2B)1-3.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.

Solubility :

DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to two weeks at 4°C or six months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

104987-11-3

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(3S,4R,5S,8R,9E,12S,14S,15R,16S,18R,19R,26aS)-5,6,8,11, 12,13,14,15,16,17,18,19,24,25,26,26a Hexadecahydro-5,19 - dihydroxy-3-[(1E)-2-[(1R,3R,4R)-4-hydroxy-3-methoxycyc lohexyl]-1- methylethenyl]-14,16-dimethoxy-4,10,12,18-te tramethyl-8-(2-propen-1-yl)- 15,19-epoxy-3H-pyrido[2,1-c ][1,4]oxaazacyclotricosine-1,7,20,21(4H,23H)tetrone

more info or order :

Alomone Labs product webpage