Forskolin | Alomone Labs F-500 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Forskolin

catalog :

F-500

more info or order :

Alomone Labs product webpage

citations: 8

Reference
He L, Li Y, Zeng N, Stiles B. Regulation of basal expression of hepatic PEPCK and G6Pase by AKT2. Biochem J. 2020;477:1021-1031 pubmed publisher
Rinaldi L, Delle Donne R, Catalanotti B, Torres Quesada O, Enzler F, Moraca F, et al. Feedback inhibition of cAMP effector signaling by a chaperone-assisted ubiquitin system. Nat Commun. 2019;10:2572 pubmed publisher
Brueggemann L, Cribbs L, Schwartz J, Wang M, Kouta A, Byron K. Mechanisms of PKA-Dependent Potentiation of Kv7.5 Channel Activity in Human Airway Smooth Muscle Cells. Int J Mol Sci. 2018;19: pubmed publisher
Paci P, Gabriele S, Ris L. A new method allowing long-term potentiation recordings in hippocampal organotypic slices. Brain Behav. 2017;7:e00692 pubmed publisher
Palma A, Galizia L, Kotsias B, Marino G. CFTR channel in oocytes from Xenopus laevis and its regulation by xShroom1 protein. Pflugers Arch. 2016;468:871-80 pubmed publisher
García Escudero V, ROSALES M, Muñoz J, Scola E, Medina J, Khalique H, et al. Patient-derived olfactory mucosa for study of the non-neuronal contribution to amyotrophic lateral sclerosis pathology. J Cell Mol Med. 2015;19:1284-95 pubmed publisher
SvalÃ¸ J, Sheykhzade M, Nordling J, Matras C, Bouchelouche P. Functional and molecular evidence for Kv7 channel subtypes in human detrusor from patients with and without bladder outflow obstruction. PLoS ONE. 2015;10:e0117350 pubmed publisher
Villers A, Godaux E, Ris L. Long-lasting LTP requires neither repeated trains for its induction nor protein synthesis for its development. PLoS ONE. 2012;7:e40823 pubmed publisher

image

image 1 :

image 2 :

Alomone Labs Forskolin activates (in vivo)adenylate cyclase in NIH/C6 neuroblastoma cells. - Cells were pre-incubated for 30 min with IBMX (10 mM) and then stimulated for 30 min with different concentrations ofForskolin(#F-500). Cellular cAMP levels were measured by an ELISA kit.

product information

CAT :

F-500

SKU :

F-500_10 mg

Product Name :

Forskolin

Group Type :

Non Antibodies

Product Type :

S. Molecules

Accession :

https://www.uniprot.org/uniprotkb/P0C1Y9/entry

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Forskolin is a cell permeable diterpenoid isolated from Coleus forskohlii. Forskolin has a unique ability to stimulate adenylate cyclase activity and increase cyclic adenosine monophosphate (cAMP)1-10.

Supplier :

Alomone Labs

Long Description :

Forskolin (#F-500) is a highly pure, natural, and biologically active compound.

Short Description :

An Adenylate Cyclase Activator

MW :

410.5

Synonyms :

Colforsin, Coleonol

Source :

Natural

Molecular formula :

C22H34O7.

Effective Concentration :

5-10 μM.

Activity :

Forskolin increases cAMP levels by activating adenylate cyclase1.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

Solubility :

DMSO, ethanol. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four months at 4°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>97% (HPLC)

CAS No :

66575-29-9

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Cited Application :

Electrophysiology

Chemical Name :

[3R-(3a,4ab,5b,6b,6aa,10a,10ab,10ba)]-5-(Acetyloxy)-3-e thenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7,10a-pent amethyl-1H-naphtho[2,1-b]pyran-1-one.

more info or order :

Alomone Labs product webpage