Fasciculin-II | Alomone Labs F-225 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Fasciculin-II

catalog :

F-225

more info or order :

Alomone Labs product webpage

citations: 6

Reference
Bernadzki K, Daszczuk P, Rojek K, Pęziński M, Gawor M, Pradhan B, et al. Arhgef5 Binds α-Dystrobrevin 1 and Regulates Neuromuscular Junction Integrity. Front Mol Neurosci. 2020;13:104 pubmed publisher
Florkowska A, Meszka I, Zawada M, Legutko D, Proszynski T, Janczyk Ilach K, et al. Pax7 as molecular switch regulating early and advanced stages of myogenic mouse ESC differentiation in teratomas. Stem Cell Res Ther. 2020;11:238 pubmed publisher
Blotnick Rubin E, Anglister L. Fine Localization of Acetylcholinesterase in the Synaptic Cleft of the Vertebrate Neuromuscular Junction. Front Mol Neurosci. 2018;11:123 pubmed publisher
Wen H, Hubbard J, Wang W, Brehm P. Fatigue in Rapsyn-Deficient Zebrafish Reflects Defective Transmitter Release. J Neurosci. 2016;36:10870-10882 pubmed
Wen H, McGinley M, Mandel G, Brehm P. Nonequivalent release sites govern synaptic depression. Proc Natl Acad Sci U S A. 2016;113:E378-86 pubmed publisher
Sharabi O, Peleg Y, Mashiach E, Vardy E, Ashani Y, Silman I, et al. Design, expression and characterization of mutants of fasciculin optimized for interaction with its target, acetylcholinesterase. Protein Eng Des Sel. 2009;22:641-8 pubmed publisher

image

image 1 :

Alomone Labs Fasciculin-IIpotently inhibits Acetylcholinesterase (AChE) activity. - AChE activity was determined colorimetrically using Ellman's reagent as production of thiocholine from acetylthiocholine. Absorbance was measured at 405 nm after a 30 min incubation. Normalized activity of 100 mU/ml (green) and 400 mU/ml (black) human AChE as per cent of control was plotted against increasing concentrations ofFasciculin-II(#F-225) showing concentration-dependent inhibition with pIC50? 6.7-6.5.

image 2 :

Alomone Labs Chlormethiazole hydrochloride activates GABA(A) receptors expressed inXenopusoocytes. - Time course of GABA(A) ?1/?2 currents directly activated by 0.25 mM and 1 mMChlormethiazole hydrochloride(#C-360) reversibly and in a concentration-dependent manner while membrane potential was held at -60 mV.

product information

cat :

F-225

SKU :

F-225_0.5 mg

Product Name :

Fasciculin-II

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P0C1Z0

Accession Number :

https://www.uniprot.org/uniprotkb/P0C1Z0/entry

Applications :

Acetylcholinesterase (AChE) activity

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Dendroaspis angusticeps (Eastern green mamba).

Source :

Natural protein

Gene ID :

ACHE

Product Page - Scientific background :

Fasciculin-II is a 61 amino acid peptidyl toxin with four disulfide bonds, isolated from the Eastern green mamba (Dendroaspis angusticeps) venom. Fasciculin-II belongs to the three finger protein of Elapidae toxin family. Fasciculin-II differs from Fasciculin-I only at one position by a replacement of Tyr with Asp or Asn1.Fasciculin-II was shown to be a selective potent and reversible blocker of acetylcholinesterase by binding to the peripheral site of the enzyme1. Injection of Fasciculin-II to mice causes severe, generalized, and long-lasting twitching (fasciculation) due to its ability to prevent degradation and therefore promote acetylcholine accumulation at the neuromuscular junction2.

Supplier :

Alomone Labs

Target :

Acetylcholinesterase (AChE)

Long Description :

A Selective, Potent and Reversible Blocker of Acetylcholinesterase

Short Description :

A Selective, Potent and Reversible Blocker of Acetylcholinesterase

MW :

6749.6 Da

Synonyms :

Fasciculin-2, Fas-2, Fas2, FAS-II, Toxin TA1, Acetylcholinesterase toxin F-VII, fasciculin, fasciculin II, fasciculin 2

Modifications :

Disulfide bonds between: Cys3-Cys22, Cys17-Cys39, Cys41-Cys52 and Cys53-Cys59

Molecular formula :

C276H438N88O90S10

Effective Concentration :

1 - 10 nM

Activity :

Fasciculin-II selectively and potently binds and inhibits acetylcholinesterase.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥99% (HPLC)

CAS No :

95506-56-2

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

TMCYSHTTTSRAILTNCGENSCYRKSRRHPPKMVLGRGC
GCPPGDDNLEVKCCTSPDKCNY-OH

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage