chemical

(S)-(-)-5-Fluorowillardiine

F-205

F-205_10 mg

(S)-(-)-5-Fluorowillardiine

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

(S)-(-)-5-Fluorowillardiine is a highly potent and selective AMPA receptor agonist. 5-Fluorowillardiine is synthesized as two distinct isomers: R and S. (S)-(-)-5-Fluorowillardiine is selective over kainate receptors and is more potent and selective than AMPA itself; at equilibrium, (S)-(-)-5-Fluorowillardiine is seven times more potent than AMPA and 30 times more potent than willardiine. (S)-(-)-5-Fluorowillardiine demonstrates an EC50 value of 1.5 µM1,2.The ionotropic glutamate AMPA receptors (AMPARs) are the primary receptors mediating fast excitatory synaptic transmission in the mammalian brain. This function is essential for synaptic plasticity and learning and memory3,4.

Alomone Labs

AMPA receptors

(S)-(-)-5-Fluorowillardiine (#F-205) is a highly pure, synthetic, and biologically active compound.

A Highly Potent and Selective Agonist of AMPA Receptors

217.16

Fluoro-willardiine, (S)-(−)-α-Amino-5-fluoro-3,4-dihydro-2,4-dioxo-1(2H)-pyrimidinepropanoic acid

Synthetic

C7H8FN3O4.

10 nM - 1 µM.

(S)-(-)-5-Fluorowillardiine is a potent and selective AMPA receptor agonist (Ki values of 14.7 nM and 25.1 nM, on hGluR1 and hGluR2, respectively), selective over kainate receptors (Ki of 1.82 µM on hGluR5). More potent and selective than AMPA1-2.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.

Soluble up to 50 mM in 1.1eq NaOH, with gentle heating. Centrifuge all products before handling (10000 x g 5 min).

Store at -20°C. It is recommended to aliquot stock solutions to prevent repeated thawing and freezing.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>97%

140187-23-1

Lyophilized Powder

Contact Alomone Labs for technical support and product customization

41116134

Yes

(2S)-2-amino-3-(5-fluoro-2,4-dioxopyrimidin-1-yl)propanoic acid.