chemical

FITM

F-200

F-200_10 mg

FITM

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

FITM is a potent and selective antagonist of mGluR1, displaying an IC50 value of 5.1 nM1 with little inhibition of mGluR2, mGluR5, and mGluR81,2.Glutamate is the most abundant excitatory neurotransmitter in the central nervous system and it modulates activity of many types of synapses by activating in part metabotropic glutamate receptors (mGluRs), members of G-protein coupled receptors (GPCRs). These receptors are divided into three groups based on sequence homology with a total of eight subtypes, mGluR1 to mGluR8.mGluR1 plays an important role in the central sensitization of pain and in a variety of functions with potential implications in neurological and psychiatric disorders2,3.

Alomone Labs

mGluR1

FITM (#F-200) is a highly pure, synthetic, and biologically active compound.

A Potent and Selective Antagonist of mGluR1

371.43

Synthetic

C18H18FN5OS.

1-100 nM.

FITM is a potent, noncompetitive antagonist of mGluR1 receptors (IC50 = 5.1 nM), selective over mGluR5 (IC50 = 7000 nM), mGluR2 and mGluR8 (IC50 >10 μM) 1. FITM demonstrates antipsychotic-like effects in several animal models.1 The radiolabeled version [18F]FITM was successfully profiled as a PET ligand for mGluR1 imaging in rat and monkey brain2.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.

Soluble in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

-20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>99%

932737-65-0

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

4-fluoro-N-methyl-N-[4-[6-(propan-2-ylamino)pyrimidin-4-yl]-1,3-thiazol-2-yl]benzamide.