sFTX-3.3 | Alomone Labs F-130 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

sFTX-3.3

catalog :

F-130

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Louiset E, Duparc C, Lenglet S, Gomez Sanchez C, Lefebvre H. Role of cAMP/PKA pathway and T-type calcium channels in the mechanism of action of serotonin in human adrenocortical cells. Mol Cell Endocrinol. 2017;441:99-107 pubmed publisher

image

image 1 :

Alomone Labs sFTX-3.3 blocks P-type Ca2+currents inXenopusoocytes. - A. Time course of P-type channel (CaV2.1+a2d1+b1a) activity before and during applications of 500 ?MsFTX-3.3(#F-130) and upon wash. Holding potential was -80 mV and currents were elicited every 10 seconds by 100 ms step to 0 mV. B. Superimposed current traces of P-type channels before and during applications of 500 ?M sFTX-3.3 (taken from the experiment described in A).

image 2 :

product information

CAT :

F-130

SKU :

F-130_1 mg

Product Name :

sFTX-3.3

Group Type :

Non Antibodies

Product Type :

S. Molecules

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Polyamine amide spider toxins inhibit neuronal voltage-activated Ca2+ currents. A terminal arginine group is an important feature of these Ca2+ channel antagonists1-2.FTX is a polyamine component of the venom of the American funnel web spider Agelenopsis aperta, and has been described as a selective antagonist of P-type voltage-dependent Ca2+ channels3-4. However, it has been demonstrated that FTX has actions at other sites including NMDA and GABA receptors and also on T-type calcium channels5.sFTX-3.3, a synthetic funnel web spider toxin6-7, is a polyamine amide analogue of FTX. The structures of these polyamine containing compounds are not identical: sFTX-3.3 contains an amide carbonyl oxygen that is absent from the predicted structure of native FTX8. sFTX-3.3 has been shown to block P-type calcium channels in rat cerebellar Purkinje cells8 and antagonizes P-, N-, and L-type calcium channels in mammalian Purkinje and superior cervical ganglia neurons with similar potencies. Its IC50 against P-, N-, and L-type channels are ~0.24 mM, ~0.7 mM and ~0.7 mM, respectively9.

Supplier :

Alomone Labs

Target :

P-type Ca2+ channels

Long Description :

sFTX-3.3 (#F-130) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Blocker of P-Type CaV Channels

MW :

287.4

Synonyms :

Synthetic Funnel Web Spider Toxin, Arginyl polyamine

Source :

Synthetic

Molecular formula :

C12H29N7O.

Effective Concentration :

0.1-1 mM.

Activity :

sFTX-3.3, a synthetic funnel web spider toxin1-2, is a polyamine amide analogue of FTX. It blocks P-, N-, and L-type Ca2+ channels in mammalian Purkinje and superior cervical ganglia neurons with similar potencies3.

Reconstitution and Solubility :

Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>95%

CAS No :

141997-14-0

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

Pentanamide,2-amino-5-[(aminoiminomethyl)amino]-N-[3-[(3-aminopropyl)amino]propyl]-, (2S)-.

more info or order :

Alomone Labs product webpage