chemical

Endothall

E-180

E-180_5 mg

Endothall

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Endothall is a synthetic structural analogue of Cantharidin (#C-170), widely used in agriculture as a pesticide and herbicide for both terrestrial and aquatic weeds1,2.Endothall was found as a potent inhibitor of spinach leaf Protein Phosphatase 1 and Protein Phosphatase 2A but a less potent inhibitor than Cantharidin for mammalian liver Protein Phosphatase 1 and Protein Phosphatase 2A.Endothall is a more effective herbicide than Cantharidin possibly due to the expression of various Protein Phosphatases in plant or to permeability differences1,2.

Alomone Labs

Protein Phosphatase 1 and Protein Phosphatase 2A

Endothall (#E-180) is a highly pure, synthetic, and biologically active compound.

An Inhibitor of Protein Phosphatase 1 and Protein Phosphatase 2A

186.16

Synthetic

C8H10O5.

200-500 nM.

Endothall is a cantharidin analogue and potently inhibits PP2A (IC50 = 90 nM) with a slight effect on PP1 (IC50 = 5 µM)1.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.

Water. Centrifuge all product preparations before use (10000 x g 5 min).

Up to two weeks at 4°C or six months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>98%

145-73-3

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

7-Oxabicyclo[2.2.1]heptane-2,3-dicarboxylic acid.