β-Dendrotoxin | Alomone Labs D-360 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

β-Dendrotoxin

catalog :

D-360

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Manicam C, Ginter N, Li H, Xia N, Goloborodko E, Zadeh J, et al. Compensatory Vasodilator Mechanisms in the Ophthalmic Artery of Endothelial Nitric Oxide Synthase Gene Knockout Mice. Sci Rep. 2017;7:7111 pubmed publisher
Manicam C, Staubitz J, Brochhausen C, Grus F, Pfeiffer N, Gericke A. The Gatekeepers in the Mouse Ophthalmic Artery: Endothelium-Dependent Mechanisms of Cholinergic Vasodilation. Sci Rep. 2016;6:20322 pubmed publisher
Wilson S, Pappone P. P2 receptor modulation of voltage-gated potassium currents in Brown adipocytes. J Gen Physiol. 1999;113:125-38 pubmed
Zygmunt P, Edwards G, Weston A, Larsson B, Högestätt E. Involvement of voltage-dependent potassium channels in the EDHF-mediated relaxation of rat hepatic artery. Br J Pharmacol. 1997;121:141-9 pubmed

image

image 1 :

Alomone Labs ?-Dendrotoxin inhibits cloned KV1.1 and KV1.2 channel currents heterologously expressed inXenopusoocytes. - KV1.1 (left in 2 mM K+) and KV1.2 (right in 5 mM K+) channel currents elicited by 200 ms depolarization from holding potential of -100 mV to +20 mV before and during application of 100 nM?-Dendrotoxin(#B-360). 62% (n = 4) of the KV1.1 and 74% (n = 4) of the KV1.2 channels were inhibited by 100 nM ?-Dendrotoxin respectively.

image 2 :

Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate: - 1.Anti-LINGO-1 (extracellular) Antibody (#ANT-032) (1:500).2. Anti-LINGO-1 (extracellular) Antibody preincubated with the negative control antigen.

product information

cat :

D-360

SKU :

D-360_35 mcg

Product Name :

β-Dendrotoxin

Group Type :

Non Antibodies

Product Type :

Proteins

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Dendroaspis angusticeps (Eastern green mamba).

Source :

Natural protein

Gene ID :

KCNA1 ,KCNA2

Product Page - Scientific background :

β-Dendrotoxin is found in the Dendroaspis angusticeps snake venom1,2. It blocks KV1.1 and KV1.2 channels expressed in Xenopus oocytes.

Supplier :

Alomone Labs

Target :

KV1.1, KV1.2 K+ channels

Long Description :

A Potent Blocker of KV1.1 and KV1.2 Channels

Short Description :

A Potent Blocker of KV1.1 and KV1.2 Channels

MW :

~7000 Da

Effective Concentration :

10 - 500 nM

Activity :

β-Dendrotoxin inhibits 4-AP sensitive, inactivating voltage-gated K+ channels (KV1.1, KV1.2).

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage