D-3263 hydrochloride | Alomone Labs D-195 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

D-3263 hydrochloride

catalog :

D-195

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs D-3263 hydrochloride activates TRPM8 channels expressed in HEK-293 cells. - Dose response curve for the activation of human TRPM8 by increasing concentrations ofD-3263 hydrochloride(#D-195). Cells were loaded with Calcium-5 dye.Changes in intracellular Ca2+following stimulation were detected as changes in relative fluorescence (RFU). EC50was determined at 75 nM.

product information

CAT :

D-195

SKU :

D-195_10 mg

Product Name :

D-3263 hydrochloride

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Storage before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

D-3263 hydrochloride, is a potent agonist of the transient receptor potential melastatin-8 (TRPM8) channel1. Activation of the channel increases Ca2+ and Na+ entry and induces cell death in TRPM8-expressing tumor cells. This agent may decrease androstanolone (DHT) levels, which may contribute to its inhibitory effects on prostate cancer and prostatic hyperplasia1.The compound shows activity in preclinical tumor models and was tested in phase 1 clinical trials for the treatment of prostate cancer1.TRPM8 is a Ca2+-permeable channel, a member of the 'long' or melastatin (M) group of TRP proteins and is activated by temperatures by the range of 8-28°C or by cooling compounds. The TRPM8 gene is and up-regulated in prostate cancer and other malignancies. It is specifically expressed in temperature-sensing trigeminal and dorsal root ganglion neurons2,3.

Supplier :

Alomone Labs

Target :

TRPM8 channels

Long Description :

D-3263 hydrochloride (#D-195) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Potent Activator of TRPM8 Channels

MW :

409.95

Source :

Synthetic

Molecular formula :

C21H32ClN3O3.

Effective Concentration :

1 nM - 10 µM.

Activity :

D-3263 hydrochloride is a potent TRPM8 channel agonist1.It shows evidence of activity in preclinical tumor models and was tested in phase 1 clinical trials for the treatment of prostate cancer1-3.

Reconstitution and Solubility :

Up to 100 mM in DMSO. Centrifuge all products before use (10000 x g 5 min).

Solubility :

Up to 100 mM in DMSO. Centrifuge all products before use (10000 x g 5 min).

Storage of solutions :

Up to three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>97%

CAS No :

1008763-54-9

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

3-(2-aminoethyl)-5-methoxy-1-[(1R,2S,5R)-5-methyl-2-propan-2-ylcyclohexanecarbonyl]benzimidazol-2-one; hydrochloride.

more info or order :

Alomone Labs product webpage