chemical

5-(N,N-Dimethyl)amiloride hydrochloride

D-165

D-165_10 mg

5-(N,N-Dimethyl)amiloride hydrochloride

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

5-(N,N-Dimethyl)amiloride hydrochloride (DMA) is an amiloride derivative that selectively inhibits Na+/H+ exchangers in cellular membranes, resulting in the retention of H+ ions and in the decrease of intracellular pH. Amiloride derivatives are commonly used for their diuretic and antihypertensive properties.Several studies have reported that DMA has positive effects in pancreatic β-cells on insulin release as a result of mis-regulation of intracellular pH and enhanced insulin secretion. Thus, amiloride derivatives are promising potential therapeutic agents for Type 2 Diabetes. In addition, positive effects on other cell functions have been demonstrated such as, cell membrane electrical activity, increase in intracellular Ca2+ and retention of K+1. DMA also enables glucose to induce time-dependent potentiation and enables it to occur in situations where it is normally absent1,2. Another study has suggested the possibility of DMA protection of the heart against post-resuscitation myocardial injury3.DMA also has the ability to inhibit the Na+/Ca2+ exchanger and to increase its currents in rat cardiomyocytes. Experimental trials have showed that DMA increases calcium sensitivity in normal and hypertrophied cardiomyocytes4.DMA also blocks TRPA1 channels5.

Alomone Labs

TRPA1 channel, Na+/H+ and Na+/Ca2+ exchangers

5-(N,N-Dimethyl)amiloride hydrochloride (#D-165) is a highly pure, synthetic, and biologically active compound.

A Blocker of TRPA1 Channels and Selective Blocker of Na+/H+ and Na+/Ca2+ Exchangers

294.1

DMA, Dimethyl amiloride

Synthetic

C8H13Cl2N7O.

1-100 µM.

5-(N,N-Dimethyl)amiloride hydrochloride blocks TRPA1 channels with an IC50 of 80 µM1.

Up to 50 mM in methanol. Centrifuge all product preparations before use (5,000 x g for 1 min).

Up to 50 mM in methanol. Centrifuge all product preparations before use (5,000 x g for 1 min).

Up to four weeks at 4°C or three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>99% (HPLC)

1214-79-5

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

3-amino-6-chloro-N-(diaminomethylidene)-5-(dimethylamino)pyrazine-2-carboxamide;hydrochloride.