chemical

1,1-Dimethyl-4-phenylpiperazinium iodide

D-125

D-125_1 g

1,1-Dimethyl-4-phenylpiperazinium iodide

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels comprising five subunits. The muscle-type receptor is made of two α1, β, γ and δ subunits, while the neuronal nicotinic receptors are formed by different combinations of two α and three β subunits. These receptors are expressed on neuronal and different types of non-neuronal cells such as, lymphocytes, alveolar macrophages, airway smooth muscle cells, epithelial cells, and fibroblasts1.nAChRs mediate numerous specific physiological functions depending on the type of cell involved. For example, nicotine induces the release of dopamine in the central nervous system and stimulates nitric oxide (NO) synthase gene expression in endothelial cells2.1,1-Dimethyl-4-phenylpiperazinium iodide (DMPP) is a selective nAChR agonist. For a long time, DMPP was considered to be the prototype of ganglionic stimulating agent, since it was able to activate the nicotinic receptor in sympathetic ganglia with higher potency than the muscle-type. However, it has since been shown that DMPP binds with higher affinity to the nicotinic receptor in the brain3. DMPP activates α4 nicotinic channels expressed in Xenopus oocytes with IC50 of 0.77 uM4.Nicotinic agonists, such as DMPP, have anti-inflammatory effects in mouse models of hypersensitivity pneumonitis, asthma, airway inflammation, and Type I diabetes5. DMPP reduces the release of IL-1 and TNF from isolated spleen cells, and the release of TNF and IL-6 from macrophages6.

Alomone Labs

nAChR

1,1-Dimethyl-4-phenylpiperazinium iodide (#D-125) is a highly pure, synthetic, and biologically active compound.

An Agonist of nAChR

318.2

DMPP

Synthetic

C12H19IN2.

0.5-200 µM.

DMPP activates α4 nicotinic channels expressed in Xenopus oocytes with IC50 0.77 μM1.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.

Water. Centrifuge all product preparations before use (10000 x g 5 min).

Up to four weeks at 4°C or three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>98%

54-77-3

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

1,1-Dimethyl-4-phenylpiperazinium iodide.