chemical

DMAB-anabaseine dihydrochloride

D-110

D-110_10 mg

DMAB-anabaseine dihydrochloride

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

nAChRs are members of the Cys-loop ligand-gated ion channel superfamily, located both in the peripheral and central nervous systems (PNS and CNS, respectively). These receptors, existing as both homopentameric and heteropentameric transmembrane ion channels, are validated therapeutic targets for various CNS pathologies1.DMAB-anabaseine (DMAB), an anabaseine analog, is a selectively partial agonist at α7 receptors which are predominantly found in the CNS2,3. In contrast, DMAB is an antagonist at α3β4, α4β2 and other nicotinic receptors4,5 and blocks the α4β2 current with IC50 ~5 µM6.

Alomone Labs

nAChR

DMAB-anabaseine dihydrochloride (#D-110) is a highly pure, synthetic, and biologically active compound.

Antagonist or Partial Agonist of nAChR

364.3

3-(4)-Dimethylaminobenzylidine anabaseine

Synthetic

C19H21N3*2HCl.

0.1-10 µM.

DMAB-anabaseine dihydrochloride is a partial agonist of α7 nicotinic acetylcholine receptors (nAChR) and antagonist of α4/β2 nAChR1. It blocks the acetylcholine induced α7 current with IC50 ~5 µM2. DMAB-anabaseine dihydrochloride can also displace isotopic (-)-cytisine and α-bungarotoxin binding to rat brain membranes with high affinity3.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.

Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).

Up to one week at 4°C or six months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>98%

154149-38-9

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

4-[(5,6-Dihydro[2,3'-bipyridin]-3(4H)-ylidene)methyl]-N,N-dimethylbenzenamine dihydrochloride.