Cycloheximide | Alomone Labs C-850 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Cycloheximide

catalog :

C-850

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone LabsCycloheximide inhibits green fluorescent protein (GFP) synthesis in HEK 293 cells. - Cells were transiently transfected with GFP for 18 h followed by treatment with (A) or without (B) 100 MCycloheximide(#C-850). Cells were visualized under fluorescent microscopy 24 h later.

image 2 :

Peptide (C)RNRTV(S)YDLSPPILS corresponding to amino acid residues 172 - 186 of mouse P2RY6 (Accession Q9ERK9). 2nd extracellular loop.

product information

CAT :

C-850

SKU :

C-850_1 g

Product Name :

Cycloheximide

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Storage before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Cycloheximide is an antibiotic which is primarily used for the isolation of dermatophytes from clinical materials.1,2 It induces death of eukaryotic cells in an apoptosis-independent pathway. Cycloheximide acts as an elongation inhibitor during protein synthesis in eukaryotes, but not prokaryotes, by competing with the binding of ATP to the 60S ribosomal subunit. Mutations in the yeast gene CYH2 that encodes L29, a component of the 60S ribosomal subunit, can lead to cycloheximide resistance.3-6

Supplier :

Alomone Labs

Long Description :

Cycloheximide (#C-850) is a highly pure, natural, and biologically active compound.

Short Description :

An Inhibitor of Protein Synthesis

MW :

281.3

Synonyms :

Actidione, Naramycin A

Source :

Natural

Molecular formula :

C15H23NO4.

Effective Concentration :

10-100 µM.

Activity :

Cycloheximide is an antibiotic which inhibits protein synthesis in eukaryotes, but not in prokaryotes, by blocking translation elongation1,2.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

Solubility :

Water, DMSO, ethanol, methanol, acetone. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

66-81-9

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

4-[2-(3,5-Dimethyl-2-oxo-cyclohexyl)-2-hydroxyethyl]-2,6-piperidinedione.

more info or order :

Alomone Labs product webpage