ω-Conotoxin MVIIA | Alomone Labs C-670 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

ω-Conotoxin MVIIA

catalog :

C-670

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Richards K, Milligan C, Richardson R, Jancovski N, Grunnet M, Jacobson L, et al. Selective NaV1.1 activation rescues Dravet syndrome mice from seizures and premature death. Proc Natl Acad Sci U S A. 2018;115:E8077-E8085 pubmed publisher
Nimmrich V, Grimm C, Draguhn A, Barghorn S, Lehmann A, Schoemaker H, et al. Amyloid beta oligomers (A beta(1-42) globulomer) suppress spontaneous synaptic activity by inhibition of P/Q-type calcium currents. J Neurosci. 2008;28:788-97 pubmed publisher
Boesmans W, Gomes P, Janssens J, Tack J, Vanden Berghe P. Brain-derived neurotrophic factor amplifies neurotransmitter responses and promotes synaptic communication in the enteric nervous system. Gut. 2008;57:314-22 pubmed

image

image 1 :

Peptide (C)REYSPAATTAENG corresponding to amino acid residues 7-19 of rat ATP1A2(AccessionP06686). Intracellular N-terminus.

image 2 :

Alomone Labs ?-Conotoxin MVIIA inhibits CaV2.2 heterologously expressed inXenopusoocytes. - A. Time course of?-Conotoxin MVIIA(#C-670) blocking action on CaV2.2 channels maximum current (expressing ?1B + ?2?1 + ?1 subunits). Maximum current amplitudes were plotted as a function of time. Membrane potential was held at -100 mV and oocytes were stimulated by a 100 ms voltage ramp to 60 mV. 50 nM ?-Conotoxin MVIIA was perfused as indicated by the bar (green) for 180 sec. B. Superimposed examples of CaV2.2 channel peak current in the absence (control) and presence (green) of 50 nM ?-Conotoxin MVIIA (taken from the experiment in A).

product information

cat :

C-670

SKU :

C-670_0.1 mg

Product Name :

ω-Conotoxin MVIIA

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P05484

Accession Number :

https://www.uniprot.org/uniprotkb/P05484/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus magus (Magical cone)

Source :

Synthetic peptide

Gene ID :

CACNA1B

Product Page - Scientific background :

ω-Conotoxin MVIIA is a synthetic peptidyl toxin originally isolated from Conus magus snail venoM ω-Conotoxin MVIIA specifically blocks CaV2.2 (α1B, N-type) channels1. The effect of the toxin is modulated by CaVβ auxiliary subunit2 and by voltage (i.e. it is more potent for inactivated channels)3.ω-Conotoxin MVIIA inhibits K+-induced 3H-GABA release in hippocampus in vivo4. This effect was with high affinity (50% block, 200 nM). The toxin was used to inhibit synaptic transmission in several peripheral preparations5,6,7. The toxin binds with high affinity to rat neocortical membranes8. It blocked cloned CaV2.2 channels transiently expressed in tsa-2019 and in Xenopus oocytes3.The FDA recently approved Prialt (synthetic ω-Conotoxin MVIIA) to treat severe chronic pain in patients who are intolerant of or refractory to other treatments such as systemic analgesics or other pain medication such as opiates. This is the first ion channel blocker to be marketed for pain.

Supplier :

Alomone Labs

Target :

CaV2.2 Ca2+ channel

Long Description :

A Specific Blocker of CaV2.2 Channel

Short Description :

A Specific Blocker of CaV2.2 Channel

MW :

2639.2 Da

Synonyms :

SNX-111, Omega-conotoxin MVIIA, Ziconotide

Modifications :

Disulfide bonds between: Cys1-Cys16, Cys8-Cys20 and Cys15-Cys25 Cys25 - C-terminal amidation

Molecular formula :

C102H172N36O32S7

Effective Concentration :

100 nM - 2 µM

Activity :

ω-Conotoxin MVIIA specifically blocks N-type CaV channels1 and also inhibits K+-induced 3H-GABA release in the hippocampus in vivo2.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥99% (HPLC)

CAS No :

107452-89-1

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

CKGKGAKCSRLMYDCCTGSCRSGKC-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage