Chelerythrine chloride | Alomone Labs C-400 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Chelerythrine chloride

catalog :

C-400

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Garcia N, Balañà C, Lanuza M, Tomas M, Cilleros Mañé V, Just Borràs L, et al. Opposed Actions of PKA Isozymes (RI and RII) and PKC Isoforms (cPKCβI and nPKCε) in Neuromuscular Developmental Synapse Elimination. Cells. 2019;8: pubmed publisher
Mohan S, Nath M, Biala Y, Yaari Y. Regulation of neuronal Na+/K+-ATPase by specific protein kinases and protein phosphatases. J Neurosci. 2019;: pubmed publisher
Libster A, Title B, Yarom Y. Corticotropin-releasing factor increases Purkinje neuron excitability by modulating sodium, potassium, and Ih currents. J Neurophysiol. 2015;114:3339-50 pubmed publisher
Hoggard J, Fan J, Lu Z, Lu Q, Sutton L, Chen Y. Claudin-7 increases chemosensitivity to cisplatin through the upregulation of caspase pathway in human NCI-H522 lung cancer cells. Cancer Sci. 2013;104:611-8 pubmed publisher

image

image 1 :

Alomone Labs Chelerythrine chloride inhibits PKC in NIH-3T3 cells. - Cells were incubated with different concentrations ofChelerythrine chloride(#C-400) for 30 min and then stimulated withPMA(#P-800) (3 ng/ml) for 20 min. Cell proteins were resolved by SDS-PAGE and probed with anti-phospho (ser)-MARCKS (myristoylated alanin-rich protein kinase C substrate).

image 2 :

Western blot analysis of human neuroblastoma (SH-SY5Y) (lanes 1 and 4) human brain astrocytoma (CCF-STTGI) (lanes 2 and 5) and rat pheochromocytoma (PC12) (lanes 3 and 6): - 1-3.Anti-CRF1/CRHR1 (extracellular) Antibody(#ACR-050) (1:200).4-6. Anti-CRF1/CRHR1 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

C-400

SKU :

C-400_1 mg

Product Name :

Chelerythrine chloride

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.

Storage before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Chelerythrine is a potent, cell-permeable inhibitor of protein kinase C (IC50 = 660 nM) that binds to the catalytic domain of PKC. Chelerythrine is at least 100-fold more selective for PKCs than for other kinases.1,2In platelets, Chelerythrine inhibits thromboxane formation and phosphoinositide metabolism.3,4 It has an inhibitory effect on ACh-induced current in PC12 cells.5Chelerythrine induces apoptotic cell death in polymorphonuclear leukocyte through activation of caspase-3.6 Furthermore, Chelerythrine treated cells underwent apoptosis, with features that suggest involvement of the mitochondrial pathway, and through a mechanism that involves inhibition of the anti-apoptotic inhibitor BclXL.7Chelerythrine inhibits SERCA pumps with an IC50 of 1.5 µM8. It also antagonizes P2X7 receptors; it inhibits ATP-induced 86Rb+ efflux with an IC50 of 5.6 µM9.

Supplier :

Alomone Labs

Target :

PKC, SERCA pumps, P2X7 receptor

Long Description :

Chelerythrine chloride (#C-400) is a highly pure, natural, and biologically active compound.

Short Description :

An Inhibitor of Protein Kinase C, SERCA Pump, and P2X7 Receptor

MW :

383.84

Source :

Natural

Molecular formula :

C21H18ClNO4.

Effective Concentration :

1-5 µM.

Activity :

Chelerythrine chloride is a potent, cell-permeable inhibitor of protein kinase C (PKC). It is at least 100-fold more selective for PKCs than for other kinases1,2.

Reconstitution and Solubility :

Water or DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

Water or DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to two weeks at 4°C or six months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99%

CAS No :

3895-92-9

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

1,2-Dimethoxy-12-methyl[1,3]benzodioxolo[5,6-c]phenanthridinium chloride.

more info or order :

Alomone Labs product webpage