chemical

Camptothecin

C-330

C-330_100 mg

Camptothecin

Non Antibodies

S. Molecules

https://www.uniprot.org/uniprotkb/P00982/entry

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Camptothecin is an anti-cancer alkaloid that specifically inhibits DNA topoisomerase I and DNA topoisomerase II (topoisomerase I/II).1-3 The highly conserved region around Gly505 of topoisomerase I was found to be important for camptothecin activity.4Camptothecin converts topoisomerase into a cell poison by trapping the cleavable covalent complex of enzyme with duplex DNA and blocking the re-ligation step. Thereby enhancing and stabilizing the formation of DNA breakage which is responsible for cell death.1-9Camptothecin inhibits (IC50 = 30 nM) Tat protein-mediated transactivation of HIV-1 and enhances PKCa activity during apoptosis in HL60 myeloid leukemia cells via a caspase-regulated mechanism.9,10

Alomone Labs

Camptothecin (#C-330) is a highly pure, natural, and biologically active compound.

Topoisomerase Inhibitor

348.36

Natural

C20H16N2O4.

1-300 nM.

Camptothecin is an alkaloid that specifically inhibits DNA topoisomerase I/II and is an anticancer agent1,2. It also promotes apoptosis in a caspase-dependent manner3.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Up to two weeks at 4°C or six months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>99%

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Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

(S)-4-Ethyl-4-hydroxy-1H-pyrano-[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione.