µ-Conotoxin KIIIA | Alomone Labs C-280 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

µ-Conotoxin KIIIA

catalog :

C-280

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs ?-Conotoxin KIIIA differentially inhibits NaV1.4 and NaV1.2 channels heterologously expressed inXenopusoocytes. - A. Time course of?-Conotoxin KIIIA(#C-280) action on NaV1.4 channels. Current amplitudes were plotted as a function of time. Oocyte membrane potential was held at -100 mV and 50 ms ramp to +50 mV was delivered every 10 seconds. 200 nM ?-Conotoxin KIIIA was perfused during the period marked in blue (100 seconds exposure). B. Example traces of NaV1.4 channel currents before (black) and during (blue) ?-Conotoxin KIIIA application. C. Time course of ?-Conotoxin KIIIA action on NaV1.2 channels. 1000 nM ?-Conotoxin KIIIA were perfused during the period marked in green (1000 seconds exposure). D. Example traces of NaV1.2 channel currents before (black) and during (green) ?-Conotoxin KIIIA application. E. Dose-response curves for NaV1.4 and NaV1.2 inhibition (upon 100 seconds exposure to the toxin; such as in A.).

product information

cat :

C-280

SKU :

C-280_0.1 mg

Product Name :

µ-Conotoxin KIIIA

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P0C195

Accession Number :

https://www.uniprot.org/uniprotkb/P0C195/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus kinoshitai (Kinoshita's cone)

Source :

Synthetic peptide

Gene ID :

SCN1A,SCN2A,SCN3A,SCN4A,SCN8A,SCN9A

Product Page - Scientific background :

µ-Conotoxin KIIIA is a toxin originally isolated from the venom of Conus Kinoshitai1. µ-Conotoxin KIIIA specifically, and in some cases reversibly, blocks TTX-sensitive mammalian voltage-dependent Na+ (NaV) channels2. In addition, µ-Conotoxin KIIIA was found to act as an effective analgesic in an inflammatory pain assay in mice in vivo2,3.

Supplier :

Alomone Labs

Target :

TTX-sensitive Na+ channels

Long Description :

A Potent Blocker of Mammalian TTX-Sensitive NaV1 Channels

Short Description :

A Potent Blocker of Mammalian TTX-Sensitive NaV1 Channels

MW :

1884 Da

Synonyms :

Mu-conotoxin KIIIA

Modifications :

Disulfide bonds between: Cys1-Cys9, Cys2-Cys15 and Cys4 -Cys16 Cys16 - C-terminal amidation

Molecular formula :

C70H106N28O22S6

Effective Concentration :

0.1 - 10 μM

Activity :

μ-Conotoxin KIIIA specifically and in some cases reversibly blocks TTX-sensitive mammalian voltage-dependent Na+ (NaV) channels1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥99% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

CCNCSSKWCRDHSRCC-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

Cited Application :

Electrophysiology

more info or order :

Alomone Labs product webpage