(±)-CPP | Alomone Labs C-175 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

(±)-CPP

catalog :

C-175

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Spratt P, Ben Shalom R, Keeshen C, Burke K, Clarkson R, Sanders S, et al. The Autism-Associated Gene Scn2a Contributes to Dendritic Excitability and Synaptic Function in the Prefrontal Cortex. Neuron. 2019;: pubmed publisher
Winland C, Welsh N, Sepulveda Rodriguez A, Vicini S, Maguire Zeiss K. Inflammation alters AMPA-stimulated calcium responses in dorsal striatal D2 but not D1 spiny projection neurons. Eur J Neurosci. 2017;46:2519-2533 pubmed publisher
Yang H, Xu Friedman M. Developmental mechanisms for suppressing the effects of delayed release at the endbulb of Held. J Neurosci. 2010;30:11466-75 pubmed publisher

image

image 1 :

Alomone Labs()-CPP inhibits NMDA (NR1+NR2A) channels expressed inXenopusoocytes. - A. Time course of NMDA currents elicited by 10 M glutamate every 50 sec while membrane potential was held at -80 mV. 1 and 10 M()-CPP(#C-175) applied for 4 min reversibly inhibited current amplitude. B. Superimposed current traces taken from experiment described in A.

image 2 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4)brain lysates: - 12. Anti-Connexin-29 (extracellular) Antibody (#ACC-223) (1:200).34. Anti-Connexin-29 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

C-175

SKU :

C-175_10 mg

Product Name :

(±)-CPP

Group Type :

Non Antibodies

Product Type :

S. Molecules

Accession :

https://www.uniprot.org/uniprotkb/P20294/entry#function

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Storage before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

(±)-CPP is a competitive, highly selective N-methyl-D-aspartate (NMDA) receptor antagonist.CPP inhibits most NMDA receptor subtypes by reversibly binding to the glutamate binding site. CPP originally synthesized as restricted analog of AP7 and AP5, NMDA antagonists has the ability to cross the blood-brain barrier which makes it useful experimentally and effective even when administered systemically1.The NMDA receptor is a glutamate-gated, multi-subunit channel complex that is involved in a variety of neural processes, including synaptic transmission, synaptic plasticity, and cell death. CPP has the ability to antagonize the release of acetylcholine from striatal regions evoked by NMDA1,2.Several studies have shown that systemic administration of CPP in rodent disrupts neurogenesis, learning and memory, blocks stress induced responses, produces antidepressant-like effects, interferes with addiction paradigms, and blocks both long-term potentiation and long-term depression1.Studies have established CPP as a pharmacological tool to investigate the role of NMDA receptors and the functional consequences of their impairment1.(±)-CPP IC50 values are 0.04 µM (NR2A), 0.3 µM (NR2B), 0.3 µM (NR2C)3. It also was found to reduce muscle tone and therefore serves as a muscle relaxant2.

Supplier :

Alomone Labs

Target :

NMDA receptors

Long Description :

(±)-CPP (#C-175) is a highly pure, synthetic, and biologically active compound.

Short Description :

An NMDA Receptor Antagonist

MW :

252.2

Source :

Synthetic

Molecular formula :

C8H17N2O5P.

Effective Concentration :

10 nM - 10 µM.

Activity :

(±)-CPP is a competitive NMDA receptor antagonist. IC50= 0.04 μM (NR2A), 0.3 μM (NR2B), 0.3 μM (NR2C)1.

Reconstitution and Solubility :

100 mM in H2O. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

100 mM in H2O. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>93%

CAS No :

100828-16-8

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

4-(3-phosphonopropyl)piperazine-2-carboxylic acid.

more info or order :

Alomone Labs product webpage